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TitleMolecular insights into the α6β4 nicotinic acetylcholine receptor function and ligand recognition.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 3153, Year 2025
Publish dateApr 2, 2025
AuthorsJiawei Su / Zhuoya Yu / Zhengji Yin / Zixuan Zhang / Jun Zhao / Yufei Meng / Renjie Li / Yiwei Gao / Hongwei Zhang / Rilei Yu / Yan Zhao /
PubMed AbstractThe α6β4 nicotinic acetylcholine receptor (nAChR) is found in the sensory neurons of dorsal root ganglia. It is a promising therapeutic target for pain. However, the difficultly of heterologous ...The α6β4 nicotinic acetylcholine receptor (nAChR) is found in the sensory neurons of dorsal root ganglia. It is a promising therapeutic target for pain. However, the difficultly of heterologous functional expression of α6β4 receptor has hindered the discovery of drugs that target it. Here, we functionally express the human α6β4 receptor and determine the cryo-EM structures of α6β4 receptor in complex with its agonists, nicotine and the preclinical drug tebanicline. These structures were captured in non-conducting desensitized states. We elucidate that the stoichiometry of α- and β- subunits in the α6β4 receptor is 2α6:3β4. Furthermore, we identify the binding pockets for nicotine and tebanicline, demonstrating the essential residues contributing to ligand affinity and providing detailed molecular insights into why these agonists have different binding affinities despite both occupying the orthosteric site of the α6β4 receptor. These structures offer significant molecular insight into the function and ligand recognition of α6β4 receptor.
External linksNat Commun / PubMed:40175361 / PubMed Central
MethodsEM (single particle)
Resolution3.25 - 3.32 Å
Structure data

60400

8zrn

EMDB-60400, PDB-8zrn:
Structure of abt
Method: EM (single particle) / Resolution: 3.25 Å

60401

8zrp

EMDB-60401, PDB-8zrp:
Structure of nico
Method: EM (single particle) / Resolution: 3.32 Å

Chemicals

PDB-1d8v:
THE RESTRAINED AND MINIMIZED AVERAGE NMR STRUCTURE OF MAP30.

ChemComp-HOH:
WATER

ChemComp-NCT:
(S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE / alkaloid*YM

Source
  • homo sapiens (human)
  • escherichia coli (E. coli)
KeywordsMEMBRANE PROTEIN / abt-bound / nico-bound

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