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TitleEstablishing a universal IVRP method for quadrivalent HPV vaccines to replace in vivo potency tests.
Journal, issue, pagesNPJ Vaccines, Vol. 10, Issue 1, Page 55, Year 2025
Publish dateMar 22, 2025
AuthorsJinpan Hu / Zijing Jia / Meng Wang / Lingling Nie / Wangjun Fu / Qingfeng Zhang / Haiyang Qin / Jianhui Nie / Xiaoyu Xu / Lingjie Xu / Fengze Wang / Yingping Chen / Bo Xing / Tao Li / Danfeng Li / Shaowei Li / Ningshao Xia / Xiangxi Wang / Weijin Huang /
PubMed AbstractSeveral human papillomavirus (HPV) L1-based virus-like particle (VLP) vaccines are in development to meet future global vaccination needs. Type-specific monoclonal antibodies with good reactivity to ...Several human papillomavirus (HPV) L1-based virus-like particle (VLP) vaccines are in development to meet future global vaccination needs. Type-specific monoclonal antibodies with good reactivity to all types of vaccines are urgently needed to evaluate vaccine potency. In this study, binding activity, neutralizing activity, conformational sensitivity, immunodominance in human serum, and versatility were compared among antibodies. A broad-spectrum binding antibody (C4-F5-127) was selected as the capture antibody; four type-specific neutralizing antibodies (6-F5-77, 11-F5-187, 16-F5-196, and 18-F5-203) were selected as detection antibodies for HPV6, 11, 16, and 18, respectively. These antibodies formed a standardized and universal in vitro relative potency (IVRP) assay kit. High-resolution cryo-electron microscopy (cryo-EM) structures of HPV6-6-F5-77, HPV11-11-F5-187, HPV16-16-F5-196 and HPV18-18-F5-203 complexes define the location and nature of epitopes, revealing serotype specific binding modes and neutralization mechanisms. The IVRP results were correlated with potency data from mouse models, offering an efficient alternative to in vivo potency experiments.
External linksNPJ Vaccines / PubMed:40118915 / PubMed Central
MethodsEM (single particle)
Resolution2.86 - 4.3 Å
Structure data

39193

8yef

EMDB-39193, PDB-8yef:
HPV6 L1 pentamer in complex with Fab F5-77
Method: EM (single particle) / Resolution: 4.3 Å

39194

8yeg

EMDB-39194, PDB-8yeg:
HPV11 L1 pentamer in complex with Fab F5-187
Method: EM (single particle) / Resolution: 3.1 Å

39195

8yeh

EMDB-39195, PDB-8yeh:
HPV16 L1 pentamer in complex with Fab F5-196
Method: EM (single particle) / Resolution: 2.86 Å

39196

8yei

EMDB-39196, PDB-8yei:
HPV18 L1 pentamer in complex with Fab F5-203
Method: EM (single particle) / Resolution: 2.93 Å

Source
  • homo sapiens (human)
  • alphapapillomavirus 10
  • human papillomavirus 11
  • Human papillomavirus 16
  • human papillomavirus type 16
  • human papillomavirus 18
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / HPV / L1 / antibody / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex / HPV16

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