EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of Nipah virus RNA synthesis.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 2261, Year 2025
掲載日	2025年3月6日
著者	Fernanda A Sala / Katja Ditter / Olexandr Dybkov / Henning Urlaub / Hauke S Hillen /
PubMed 要旨	Nipah virus (NiV) is a non-segmented negative-strand RNA virus (nsNSV) with high pandemic potential, as it frequently causes zoonotic outbreaks and can be transmitted from human to human. Its RNA- ...Nipah virus (NiV) is a non-segmented negative-strand RNA virus (nsNSV) with high pandemic potential, as it frequently causes zoonotic outbreaks and can be transmitted from human to human. Its RNA-dependent RNA polymerase (RdRp) complex, consisting of the L and P proteins, carries out viral genome replication and transcription and is therefore an attractive drug target. Here, we report cryo-EM structures of the NiV polymerase complex in the apo and in an early elongation state with RNA and incoming substrate bound. The structure of the apo enzyme reveals the architecture of the NiV L-P complex, which shows a high degree of similarity to other nsNSV polymerase complexes. The structure of the RNA-bound NiV L-P complex shows how the enzyme interacts with template and product RNA during early RNA synthesis and how nucleoside triphosphates are bound in the active site. Comparisons show that RNA binding leads to rearrangements of key elements in the RdRp core and to ordering of the flexible C-terminal domains of NiV L required for RNA capping. Taken together, these results reveal the first structural snapshots of an actively elongating nsNSV L-P complex and provide insights into the mechanisms of genome replication and transcription by NiV and related viruses.
リンク	Nat Commun / PubMed:40050611 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 2.8 Å
構造データ	51402 9gjt EMDB-51402, PDB-9gjt: Structure of Nipah Virus RNA Polymerase Complex - Apo state 手法: EM (単粒子) / 解像度: 2.6 Å 51403 9gju EMDB-51403, PDB-9gju: Structure of replicating Nipah Virus RNA Polymerase Complex - RNA-bound state 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-51722: Consensus map - Structure of replicating Nipah Virus RNA Polymerase Complex - RNA-bound state 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-51723: L - RdRp domain - focused refinement of the Nipah virus RNA polymerase complex in the RNA-bound state. 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-51724: Phosphoprotein - focused refinement of the Nipah virus RNA polymerase complex in the RNA-bound state. 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-51725: C-Terminal - focused refinement of the Nipah virus RNA polymerase complex in the RNA-bound state. 手法: EM (単粒子) / 解像度: 2.8 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-GNP: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / Gpp(NH)p / GppNHp, GMPPNP, エネルギー貯蔵分子類似体YM ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	henipavirus nipahense (ウイルス)
キーワード	VIRAL PROTEIN / RNA Polymerase / Nipah Virus / negative strand RNA Virus