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TitleCytoplasmic ribosomes on mitochondria alter the local membrane environment for protein import.
Journal, issue, pagesJ Cell Biol, Vol. 224, Issue 4, Year 2025
Publish dateApr 7, 2025
AuthorsYa-Ting Chang / Benjamin A Barad / Juliette Hamid / Hamidreza Rahmani / Brian M Zid / Danielle A Grotjahn /
PubMed AbstractMost of the mitochondria proteome is nuclear-encoded, synthesized by cytoplasmic ribosomes, and targeted to the mitochondria posttranslationally. However, a subset of mitochondrial-targeted proteins ...Most of the mitochondria proteome is nuclear-encoded, synthesized by cytoplasmic ribosomes, and targeted to the mitochondria posttranslationally. However, a subset of mitochondrial-targeted proteins is imported co-translationally, although the molecular mechanisms governing this process remain unclear. We employ cellular cryo-electron tomography to visualize interactions between cytoplasmic ribosomes and mitochondria in Saccharomyces cerevisiae. We use surface morphometrics tools to identify a subset of ribosomes optimally oriented on mitochondrial membranes for protein import. This allows us to establish the first subtomogram average structure of a cytoplasmic ribosome at the mitochondrial surface in the native cellular context, which showed three distinct connections with the outer mitochondrial membrane surrounding the peptide exit tunnel. Further, this analysis demonstrated that cytoplasmic ribosomes primed for mitochondrial protein import cluster on the outer mitochondrial membrane at sites of local constrictions of the outer and inner mitochondrial membranes. Overall, our study reveals the architecture and the spatial organization of cytoplasmic ribosomes at the mitochondrial surface, providing a native cellular context to define the mechanisms that mediate efficient mitochondrial co-translational protein import.
External linksJ Cell Biol / PubMed:40047641 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution8.0 - 15.0 Å
Structure data

EMDB-48751: Structrure of cytoplasmic S. cerevisiae ribosome oriented for protein import on the outer mitochondrial membrane
Method: EM (subtomogram averaging) / Resolution: 15.0 Å

EMDB-48752: Structure of cytoplasmic 80S ribosome from S. cerevisiae
Method: EM (subtomogram averaging) / Resolution: 8.0 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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