EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of a natural circularly permuted group II intron reveal mechanisms of branching and backsplicing.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 32, Issue 6, Page 1101-1110, Year 2025
掲載日	2025年2月27日
著者	Xiaobin Ling / Yuqi Yao / Jinbiao Ma /
PubMed 要旨	Circularly permuted (CP) group II introns, identified in various bacteria phyla, swap domains D5 and D6 near the 5' end and have reversed splice sites (SSs), leading to backsplicing and circular RNA ...Circularly permuted (CP) group II introns, identified in various bacteria phyla, swap domains D5 and D6 near the 5' end and have reversed splice sites (SSs), leading to backsplicing and circular RNA formation. In this study, we present multiple high-resolution cryo-electron microscopy structures of a natural CP group II intron from Comamonas testosteroni KF-1 (Cte 1), elucidating the molecular mechanisms of branching and backsplicing. During branching, the 5' SS is positioned by an auxiliary sequence (AUX)-enhanced interaction between the exon-binding site and intron-binding site (IBS) and stacks on the branch-site adenosine within D6, allowing the attacking 2'-OH group to coordinate with a metal ion in the active center. In backsplicing, the 3' SS is aligned with the branching step, leaving IBS in the active center, stabilized by base pairing with the AUX, which enables the free 3'-end hydroxyl group to directly attack the scissile phosphate of 3' SS. Furthermore, a groove in Cte 1 may stabilize the circular RNA. These findings highlight a conserved catalytic mechanism for canonical group II introns, albeit facilitated by the versatile AUX, opening avenues for designing potent ribozymes producing circular RNAs.
リンク	Nat Struct Mol Biol / PubMed:40016344
手法	EM (単粒子)
解像度	2.63 - 3.06 Å
構造データ	38173 8x9k EMDB-38173, PDB-8x9k: Cte_Branching,Ca,inactive 手法: EM (単粒子) / 解像度: 2.63 Å 38174 8x9l EMDB-38174, PDB-8x9l: Cte-U537ins_Branch Intermediate,Ca,active 手法: EM (単粒子) / 解像度: 3.04 Å 38175 8x9m EMDB-38175, PDB-8x9m: Cte-Branch Product, Mg, post 手法: EM (単粒子) / 解像度: 2.78 Å 38176 8x9n EMDB-38176, PDB-8x9n: Cte_A695U_Branch Intermediate,Mg,inactive 手法: EM (単粒子) / 解像度: 3.01 Å 38177 8x9o EMDB-38177, PDB-8x9o: Cte-U537ins-Branching,Ca,inactive 手法: EM (単粒子) / 解像度: 3.06 Å 38182 8x9q EMDB-38182, PDB-8x9q: Cte-A695U-branching,Mg,active 手法: EM (単粒子) / 解像度: 3.04 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-K: Unknown entry / カリウムカチオン ChemComp-HOH: WATER ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	comamonas testosteroni kf-1 (バクテリア)
キーワード	RNA / SPLICING / CP group ii intron / Cte / back-splicing / circular RNA / Cryo-EM / Cte-branching / cirular RNA