EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural dynamics of human fatty acid synthase in the condensing cycle.
ジャーナル・号・ページ	Nature, Vol. 641, Issue 8062, Page 529-536, Year 2025
掲載日	2025年2月20日
著者	Wooyoung Choi / Chengmin Li / Yifei Chen / YongQiang Wang / Yifan Cheng /
PubMed 要旨	Long-chain fatty acids are the building blocks of fat in human bodies. In mammals, fatty acid synthase (FASN) contains multiple enzymatic domains to catalyse all chemical reactions needed for de novo ...Long-chain fatty acids are the building blocks of fat in human bodies. In mammals, fatty acid synthase (FASN) contains multiple enzymatic domains to catalyse all chemical reactions needed for de novo fatty acid synthesis. Although the chemical reactions carried out by these enzymatic domains are well defined, how the dimeric FASN with an open architecture continuously catalyses such reactions to synthesize a complete fatty acid remains elusive. Here, using a strategy of tagging and purifying endogenous FASN in HEK293T cells for single-particle cryo-electron microscopy studies, we characterized the structural dynamics of endogenous human FASN. We captured conformational snapshots of various functional substates in the condensing cycle and developed a procedure to analyse the particle distribution landscape of FASN with different orientations between its condensing and modifying wings. Together, our findings reveal that FASN function does not require a large rotational motion between its two main functional domains during the condensing cycle, and that the catalytic reactions in the condensing cycle carried out by the two monomers are unsynchronized. Our data thus provide a new composite view of FASN dynamics during the fatty acid synthesis condensing cycle.
リンク	Nature / PubMed:39978408 / PubMed Central
手法	EM (単粒子)
解像度	2 - 3.3 Å
構造データ	EMDB-44230: Human endogenous FASN alone - Class 1" to "Human endogenous FASN alone - Class 1 composite map 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-44231: Human endogenous FASN alone - Class 1 consensus map 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-44263: Human endogenous FASN alone - Class 1 focused modifying wing 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-44264: Human endogenous FASN alone - Class 1 focused condensing wing 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-44267: Human endogenous FASN with Orlistat - composite map 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-44268: Human endogenous FASN with Orlistat - consensus map 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-44269: Human endogenous FASN with Orlistat - focused modifying wing 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-44270: Human endogenous FASN with Orlistat - focused condensing wing 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-44295: Human endogenous FASN with 1,3-DBP - Class 2 composite map 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-44296: Human endogenous FASN with 1,3-DBP - Class 2 consensus map 手法: EM (単粒子) / 解像度: 2.3 Å EMDB-44297: Human endogenous FASN with 1,3-DPB - Class 2 focused modifying wing 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-44298: Human endogenous FASN with 1,3-DBP - Class 2 focused condensing wing 手法: EM (単粒子) / 解像度: 2.3 Å EMDB-44300: Human endogenous FASN with 1,3-DBP - Class 1 composite map 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-44301: Human endogenous FASN with 1,3-DBP - Class 1 consensus map 手法: EM (単粒子) / 解像度: 2.7 Å 44329 9b7z EMDB-44329, PDB-9b7z: Human endogenous FASN with 1,3-DBP - Class 1 focused condensing wing 手法: EM (単粒子) / 解像度: 2.5 Å 44330 9b80 EMDB-44330, PDB-9b80: Human endogenous FASN with 1,3-DBP - Class 1 focused modifying wing 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-44371: Human endogenous FASN - combined class1 modifying wing with classification 手法: EM (単粒子) / 解像度: 2.9 Å 44378 9mj9 EMDB-44378: Human endogenous FASN - combined class1 condensing wing with classification PDB-9mj9: Condensing wing of FASN with phosphopantetheine-modified partial ACP 手法: EM (単粒子) / 解像度: 2.9 Å
由来	homo sapiens (ヒト)
キーワード	BIOSYNTHETIC PROTEIN / De novo fatty acid synthesis Fatty acid synthase / TRANSFERASE