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TitleStructural and functional analysis of SAM-dependent N-methyltransferases involved in ovoselenol and ovothiol biosynthesis.
Journal, issue, pagesStructure, Vol. 33, Page 528-538.e5, Year 2025
Publish dateMay 22, 2024 (structure data deposition date)
AuthorsIreland, K.A. / Kayrouz, C.M. / Abbott, M.L. / Seyedsayamdost, M.R. / Davis, K.M.
External linksStructure / PubMed:39862859
MethodsX-ray diffraction
Resolution1.48 - 2.27 Å
Structure data

PDB-9bxh:
OvsM from Marinimicrobium koreense, a SAM-dependent N-methyltransferase involved in ovoselenol biosynthesis
Method: X-RAY DIFFRACTION / Resolution: 1.48 Å

PDB-9bxj:
OvsM from Marinimicrobium koreense, an ovoselenol-biosynthetic N-methyltransferase in complex with SAM
Method: X-RAY DIFFRACTION / Resolution: 1.56 Å

PDB-9bxk:
OvsM from Marinimicrobium koreense, an ovoselenol-biosynthetic N-methyltransferase in complex with 5-selenohistidine and SAH
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

PDB-9bxl:
OvoM from Sulfuricurvum sp. isolate STB_99, an ovothiol-biosynthetic N-methyltransferase in complex with SAM
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

PDB-9bxm:
OvoM from Sulfuricurvum sp. isolate STB_99, a SAM-dependent N-methyltransferase involved in ovothiol biosynthesis
Method: X-RAY DIFFRACTION / Resolution: 2.27 Å

PDB-9bxn:
OvoM from Sulfuricurvum sp. isolate STB_99, an ovothiol-biosynthetic N-methyltransferase in complex with 5-thiohistidine and SAH
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-SAM:
S-ADENOSYLMETHIONINE

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

PDB-1as0:
GTP-GAMMA-S BOUND G42V GIA1

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

PDB-1asz:
THE ACTIVE SITE OF YEAST ASPARTYL-TRNA SYNTHETASE: STRUCTURAL AND FUNCTIONAL ASPECTS OF THE AMINOACYLATION REACTION

Source
  • marinimicrobium koreense (bacteria)
  • sulfuricurvum sp. (bacteria)
KeywordsTRANSFERASE / ovoselenol / 5-selenohistidine / S-adenosyl-L-methionine / N-methyltransferase / ovothiol / 5-thiohistidine

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