Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Mechanistic basis of temperature adaptation in microtubule dynamics across frog species.
Journal, issue, pages	Curr Biol, Vol. 35, Issue 3, Page 612-628.e6, Year 2025
Publish date	Feb 3, 2025
Authors	Luca Troman / Ella de Gaulejac / Abin Biswas / Jennifer Stiens / Benno Kuropka / Carolyn A Moores / Simone Reber /
PubMed Abstract	Cellular processes are remarkably effective across diverse temperature ranges, even with highly conserved proteins. In the context of the microtubule cytoskeleton, which is critically involved in a ...Cellular processes are remarkably effective across diverse temperature ranges, even with highly conserved proteins. In the context of the microtubule cytoskeleton, which is critically involved in a wide range of cellular activities, this is particularly striking, as tubulin is one of the most conserved proteins while microtubule dynamic instability is highly temperature sensitive. Here, we leverage the diversity of natural tubulin variants from three closely related frog species that live at different temperatures. We determine the microtubule structure across all three species at between 3.0 and 3.6 Å resolution by cryo-electron microscopy and find small differences at the β-tubulin lateral interactions. Using in vitro reconstitution assays and quantitative biochemistry, we show that tubulin's free energy scales inversely with temperature. The observed weakening of lateral contacts and the low apparent activation energy for tubulin incorporation provide an explanation for the overall stability and higher growth rates of microtubules in cold-adapted frog species. This study thus broadens our conceptual framework for understanding microtubule dynamics and provides insights into how conserved cellular processes are tailored to different ecological niches.
External links	Curr Biol / PubMed:39798564
Methods	EM (single particle)
Resolution	3.0 - 3.6 Å
Structure data	50811 9fvj EMDB-50811, PDB-9fvj: Xenopus borealis undecorated microtubule - 15 protofilament, 3-start helix Method: EM (single particle) / Resolution: 3.2 Å 50941 9g0o EMDB-50941, PDB-9g0o: Xenopus borealis undecorated microtubule - 14 protofilament, 3-start helix Method: EM (single particle) / Resolution: 3.3 Å 50942 9g0p EMDB-50942, PDB-9g0p: Xenopus laevis undecorated microtubule - 14 protofilament, 3-start helix Method: EM (single particle) / Resolution: 3.0 Å 50943 9g0q EMDB-50943, PDB-9g0q: Xenopus laevis undecorated microtubule - 15 protofilament, 3-start helix Method: EM (single particle) / Resolution: 3.2 Å 50945 9g0r EMDB-50945, PDB-9g0r: Xenopus laevis undecorated microtubule - 15 protofilament, 4-start helix Method: EM (single particle) / Resolution: 3.1 Å 50946 9g0s EMDB-50946, PDB-9g0s: Xenopus tropicalis undecorated microtubule - 14 protofilament, 3-start helix Method: EM (single particle) / Resolution: 3.6 Å 50947 9g0t EMDB-50947, PDB-9g0t: Xenopus tropicalis undecorated microtubule - 15 protofilament, 3-start helix Method: EM (single particle) / Resolution: 3.5 Å
Chemicals	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying moleculeYM ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM ChemComp-MG: Unknown entry
Source	xenopus borealis (Kenyan clawed frog) xenopus laevis (African clawed frog) xenopus tropicalis (tropical clawed frog)
Keywords	PROTEIN FIBRIL / Microtubule Tubulin Cytoskeleton Filament