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-Structure paper
| タイトル | Capturing eukaryotic ribosome dynamics in situ at high resolution. |
|---|---|
| ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 32, Issue 4, Page 698-708, Year 2025 |
| 掲載日 | 2025年1月9日 |
 著者 | Jing Cheng / Chunling Wu / Junxi Li / Qi Yang / Mingjie Zhao / Xinzheng Zhang /  |
| PubMed 要旨 | Many protein complexes are highly dynamic in cells; thus, characterizing their conformational changes in cells is crucial for unraveling their functions. Here, using cryo-electron microscopy, 451,700 ...Many protein complexes are highly dynamic in cells; thus, characterizing their conformational changes in cells is crucial for unraveling their functions. Here, using cryo-electron microscopy, 451,700 ribosome particles from Saccharomyces cerevisiae cell lamellae were obtained to solve the 60S region to 2.9-Å resolution by in situ single-particle analysis. Over 20 distinct conformations were identified by three-dimensional classification with resolutions typically higher than 4 Å. These conformations were used to reconstruct a complete elongation cycle of eukaryotic translation with elongation factors (eEFs). We found that compact eEF2 anchors to the partially rotated ribosome after subunit rolling and hypothesize that it stabilizes the local conformation for peptidyl transfer. Moreover, open-eEF3 binding to a fully rotated ribosome was observed, whose conformational change was coupled with head swiveling and body back-rotation of the 40S subunit. |
 リンク | Nat Struct Mol Biol / PubMed:39789210 |
| 手法 | EM (単粒子) |
| 解像度 | 3.2 - 4.7 Å |
| 構造データ |  EMDB-38654: State 8a (S8a) of yeast 80S ribosome bound to 3 tRNAs and eEF1A and eEF3 during mRNA decoding  EMDB-38655: State 8 (S8) of yeast 80S ribosome bound to 3 tRNAs and eEF1A during mRNA decoding EMDB-38656: State 1 (S1) of yeast 80S ribosome bound to 2 tRNAs and eEF1A during mRNA decoding EMDB-38657, PDB-8z71:  EMDB-38658: State 2a (S2a) of yeast 80S ribosome bound to 2 tRNAs during peptidyl transfer  EMDB-38659: State 2b (S2b) of yeast 80S ribosome bound to 2 tRNAs during peptidyl transfer EMDB-38660, PDB-8xu8:  EMDB-38661: State 2d (S2d) of yeast 80S ribosome bound to compact eEF2 and 2 tRNAs during peptidyl transfer  EMDB-38662: State 2e (S2e) of yeast 80S ribosome bound to 2 tRNAs during peptidyl transfer  EMDB-38663: State 2f (S2f) of yeast 80S ribosome bound to 2 tRNAs during peptidyl transfer  EMDB-38664: State 3 (S3) of yeast 80S ribosome bound to 2 tRNAs during translocation  EMDB-38665: State 4 (S4) of yeast 80S ribosome bound to 2 tRNAs and eEF2 during translocation EMDB-38666, PDB-8yld:  EMDB-38667: State 5 (S5) of yeast 80S ribosome bound to 2 tRNAs and eEF2 during translocation EMDB-38668, PDB-8ylr:  EMDB-38669: State 7 (S7) of yeast 80S ribosome bound to 2 tRNAs and eEF2 during tranlocation  EMDB-38670: State 9 (S9) of yeast 80S ribosome bound to 2 tRNAs  EMDB-38671: State 10 (S10) of yeast 80S ribosome bound to A tRNA |
| 由来 |
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 キーワード | TRANSLATION / 80S / compact eEF2 / elongation cycle / rotated / RIBOSOME / eEF3 / eEF2 |
saccharomyces cerevisiae s288c (パン酵母)
oryctolagus cuniculus (ウサギ)