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TitleStructural insights into the mechanism of phosphate recognition and transport by XPR1.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 18, Year 2025
Publish dateJan 2, 2025
AuthorsWenhui Zhang / Yanke Chen / Zeyuan Guan / Yong Wang / Meng Tang / Zhangmeng Du / Jie Zhang / Meng Cheng / Jiaqi Zuo / Yan Liu / Qiang Wang / Yanjun Liu / Delin Zhang / Ping Yin / Ling Ma / Zhu Liu /
PubMed AbstractXPR1 is the sole protein known to transport inorganic phosphate (Pi) out of cells, a function conserved across species from yeast to mammals. Human XPR1 variants lead to cerebral calcium-phosphate ...XPR1 is the sole protein known to transport inorganic phosphate (Pi) out of cells, a function conserved across species from yeast to mammals. Human XPR1 variants lead to cerebral calcium-phosphate deposition and primary familial brain calcification (PFBC), a hereditary neurodegenerative disorder. Here, we present the cryo-EM structure of human XPR1 in both its Pi-unbound and various Pi-bound states. XPR1 features 10 transmembrane α-helices forming an ion channel-like structure, with multiple Pi recognition sites along the channel. Pathogenic mutations in two arginine residues, which line the translocation channel, disrupt Pi transport. Molecular dynamics simulations reveal that Pi ion undergoes a stepwise transition through the sequential recognition sites during the transport process. Together with functional analyses, our results suggest that this sequential arrangement allows XPR1 to facilitate Pi ion passage via a "relay" process, and they establish a framework for the interpretation of disease-related mutations and for the development of future therapeutics.
External linksNat Commun / PubMed:39747008 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 3.3 Å
Structure data

61138

9j4x

EMDB-61138, PDB-9j4x:
CryoEM structure of human XPR1 in apo state
Method: EM (single particle) / Resolution: 2.9 Å

61139

9j51

EMDB-61139, PDB-9j51:
CryoEM structure of human XPR1 in complex with phosphate in state A
Method: EM (single particle) / Resolution: 3.1 Å

61140

9j52

EMDB-61140, PDB-9j52:
CryoEM structure of human XPR1 in complex with phosphate in state B
Method: EM (single particle) / Resolution: 3.1 Å

61141

9j53

EMDB-61141, PDB-9j53:
CryoEM structure of human XPR1 in complex with phosphate in state C
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

ChemComp-PO4:
PHOSPHATE ION

Source
  • homo sapiens (human)
KeywordsTRANSPORT PROTEIN / Phosphate / Transport / XPR1

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