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TitleAltering substrate specificity of a thermostable bacterial monoamine oxidase by structure-based mutagenesis.
Journal, issue, pagesArch. Biochem. Biophys., Vol. 764, Page 110276-110276, Year 2024
Publish dateOct 23, 2024 (structure data deposition date)
AuthorsBasile, L. / Poli, C. / Santema, L.L. / Lesenciuc, R.C. / Fraaije, M.W. / Binda, C.
External linksArch. Biochem. Biophys. / PubMed:39706511
MethodsX-ray diffraction
Resolution1.6 - 2.3 Å
Structure data

PDB-9h5p:
Crystal structure of Thermoanaerobacterales bacterium monoamine oxidase in complex with putrescine
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-9h5q:
Crystal structure of Thermoanaerobacterales bacterium monoamine oxidase in complex with spermidine and its oxidation products
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-9h5z:
Crystal structure of Thermoanaerobacterales bacterium monoamine oxidase in complex with benzylamine
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-9h64:
Crystal structure of Thermoanaerobacterales bacterium monoamine oxidase in complex with n-heptylamine
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-MG:
Unknown entry

ChemComp-PUT:
1,4-DIAMINOBUTANE

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-13D:
1,3-DIAMINOPROPANE

ChemComp-4HA:
4-HYDROXYBUTAN-1-AMINIUM

ChemComp-SPD:
SPERMIDINE

ChemComp-ABN:
BENZYLAMINE

PDB-1iso:
ISOCITRATE DEHYDROGENASE: STRUCTURE OF AN ENGINEERED NADP+--> NAD+ SPECIFICITY-REVERSAL MUTANT

Source
  • thermoanaerobacterales bacterium (bacteria)
KeywordsFLAVOPROTEIN / monoamine oxidase / Thermoanaerobacterales bacterium / enzyme / putrescine / spermidine / benzylamine / n-heptylamine

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