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-Structure paper
| タイトル | The nucleocapsid architecture and structural atlas of the prototype baculovirus define the hallmarks of a new viral realm. |
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| ジャーナル・号・ページ | Sci Adv, Vol. 10, Issue 51, Page eado2631, Year 2024 |
| 掲載日 | 2024年12月20日 |
著者 | Bronte A Johnstone / Joshua M Hardy / Jungmin Ha / Anamarija Butkovic / Paulina Koszalka / Cathy Accurso / Hariprasad Venugopal / Alex de Marco / Mart Krupovic / Fasséli Coulibaly / ![]() |
| PubMed 要旨 | Baculovirus is the most studied insect virus owing to a broad ecological distribution and ease of engineering for biotechnological applications. However, its structure and evolutionary place in the ...Baculovirus is the most studied insect virus owing to a broad ecological distribution and ease of engineering for biotechnological applications. However, its structure and evolutionary place in the virosphere remain enigmatic. Using cryo-electron microscopy, we show that the nucleocapsid forms a covalently cross-linked helical tube protecting a highly compacted 134-kilobase pair DNA genome. The ends of the tube are sealed by the base and cap substructures, which share a 126-subunit hub but differ in components that promote actin tail-mediated propulsion and nuclear entry of the nucleocapsid, respectively. Unexpectedly, sensitive searches for hidden evolutionary links show that the morphogenetic machinery and conserved oral infectivity factors originated within the lineage of baculo-like viruses (class ). The unique viral architecture and structural atlas of hallmark proteins firmly place these viruses into a separate new realm, the highest taxonomy rank, and provide a structural framework to expand their use as sustainable bioinsecticides and biomedical tools. |
リンク | Sci Adv / PubMed:39693434 / PubMed Central |
| 手法 | EM (らせん対称) / EM (単粒子) |
| 解像度 | 3.06 - 6.3 Å |
| 構造データ | ![]() EMDB-43585: Structure of the AcMNPV baculovirus VP39 nucleocapsid (C14 helical reconstruction) EMDB-43586, PDB-8vwh: ![]() EMDB-43587: The base complex of the baculovirus nucleocapsid (Autographa californica multiple nucleopolyhedrovirus) EMDB-43589, PDB-8vwj: ![]() EMDB-43590: The cap complex of the baculovirus nucleocapsid (Autographa californica multiple nucleopolyhedrovirus) ![]() EMDB-43591: The cap of the AcMNPV baculovirus nucleocapsid (localised reconstruction) |
| 化合物 | ![]() ChemComp-ZN: |
| 由来 |
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キーワード | VIRAL PROTEIN / Capsid / helical assembly / Nucleocapsid / Base / baculovirus / virus / AcMNPV / VP39 |
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autographa californica multiple nucleopolyhedrovirus (オートグラファカリフォルニア核多角体病ウイルス)
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