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TitleDesigned, Programmable Protein Cages Utilizing Diverse Metal Coordination Geometries Show Reversible, pH-Dependent Assembly.
Journal, issue, pagesMacromol Rapid Commun, Vol. 46, Issue 6, Page e2400712, Year 2025
Publish dateDec 16, 2024
AuthorsNorbert Osiński / Karolina Majsterkiewicz / Zuzanna Pakosz-Stępień / Yusuke Azuma / Artur P Biela / Szymon Gaweł / Jonathan G Heddle /
PubMed AbstractThe rational design and production of a novel series of engineered protein cages are presented, which have emerged as versatile and adaptable platforms with significant applications in biomedicine. ...The rational design and production of a novel series of engineered protein cages are presented, which have emerged as versatile and adaptable platforms with significant applications in biomedicine. These protein cages are assembled from multiple protein subunits, and precise control over their interactions is crucial for regulating assembly and disassembly, such as the on-demand release of encapsulated therapeutic agents. This approach employs a homo-undecameric, ring-shaped protein scaffold with strategically positioned metal binding sites. These engineered proteins can self-assemble into highly stable cages in the presence of cobalt or zinc ions. Furthermore, the cages can be disassembled on demand by employing external triggers such as chelating agents and changes in pH. Interestingly, for certain triggers, the disassembly process is reversible, allowing the cages to reassemble upon reversal or outcompeting of triggering conditions/agents. This work offers a promising platform for the development of advanced drug delivery systems and other biomedical applications.
External linksMacromol Rapid Commun / PubMed:39676522 / PubMed Central
MethodsEM (single particle)
Resolution2.76 - 4.31 Å
Structure data

18904

8r59

EMDB-18904, PDB-8r59:
Structure of the Co(II) triggered TRAP (S33HK35H) protein cage (levo form)
Method: EM (single particle) / Resolution: 2.86 Å

18905

8r5a

EMDB-18905, PDB-8r5a:
Structure of the Co(II) triggered TRAP (S33HK35H) protein cage (dextro form)
Method: EM (single particle) / Resolution: 2.84 Å

EMDB-18906: Structure of the Zn(II) triggered TRAP (S33HK35H) protein cage (levo form)
Method: EM (single particle) / Resolution: 3.96 Å

EMDB-18907: Structure of the Zn(II) triggered TRAP (S33HK35H) protein cage (dextro form)
Method: EM (single particle) / Resolution: 4.31 Å

EMDB-18908: Structure of the Zn(II) triggered TRAP (K35C) protein cage (levo form)
Method: EM (single particle) / Resolution: 2.76 Å

EMDB-18909: Structure of the Zn(II) triggered TRAP (K35C) protein cage (dextro form)
Method: EM (single particle) / Resolution: 2.76 Å

Chemicals

ChemComp-CO:
Unknown entry

Source
  • geobacillus stearothermophilus (bacteria)
KeywordsVIRUS LIKE PARTICLE / TRAP protein / protein cage

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