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Structure paper

TitleStructural insights into a DNA polymerase reading the xeno nucleic acid HNA.
Journal, issue, pagesNucleic Acids Res., Vol. 53, Year 2025
Publish dateMar 5, 2024 (structure data deposition date)
AuthorsGutfreund, C. / Betz, K. / Abramov, M. / Coosemans, F. / Holliger, P. / Herdewijn, P. / Marx, A.
External linksNucleic Acids Res. / PubMed:39673482
MethodsX-ray diffraction
Resolution1.82 - 2.35 Å
Structure data

PDB-8s84:
KOD-H4 DNA polymerase mutant in a ternary complex with DNA/DNA and non-hydrolyzable triphosphate
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-8s87:
KOD-H4 DNA polymerase mutant - apo structure
Method: X-RAY DIFFRACTION / Resolution: 1.82 Å

PDB-9emi:
KOD-H4 DNA polymerase mutant in a ternary complex containing six HNA nucleotides and a non-hydrolyzable triphosphate
Method: X-RAY DIFFRACTION / Resolution: 2.27 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-XG4:
2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]guanosine

ChemComp-MN:
Unknown entry

ChemComp-IMD:
IMIDAZOLE

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-NA:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

Source
  • thermococcus kodakarensis kod1 (archaea)
  • synthetic construct (others)
KeywordsTRANSFERASE / Polymerase / XNA / modified nucleotides / Reverse transcriptase / apo / mutant DNA polymerase / HNA / non-hydrolyzable triphosphate

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