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TitleEvolutionary insights into the stereoselectivity of imine reductases based on ancestral sequence reconstruction.
Journal, issue, pagesNat Commun, Vol. 15, Page 10330-10330, Year 2024
Publish dateJan 3, 2023 (structure data deposition date)
AuthorsZhu, X.X. / Zheng, W.Q. / Xia, Z.W. / Chen, X.R. / Jin, T. / Ding, X.W. / Chen, F.F. / Chen, Q. / Xu, J.H. / Kong, X.D. / Zheng, G.W.
External linksNat Commun / PubMed:39609402
MethodsX-ray diffraction
Resolution2.32 - 2.57 Å
Structure data

PDB-8hwy:
Ancestral imine reductase mutant N559_M6
Method: X-RAY DIFFRACTION / Resolution: 2.32 Å

PDB-8jku:
Ancestral imine reductase N559
Method: X-RAY DIFFRACTION / Resolution: 2.57 Å

Chemicals

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-HOH:
WATER

Source
  • escherichia (bacteria)
  • synthetic construct (others)
KeywordsOXIDOREDUCTASE / ancestral enzyme / NADP+ / DE NOVO PROTEIN / ancestral protein / NADPH-dependent oxidoreductase

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