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Structure paper

TitleLayered entrenchment maintains essentiality in the evolution of Form I Rubisco complexes.
Journal, issue, pagesEmbo J., Vol. 44, Page 269-280, Year 2025
Publish dateSep 25, 2023 (structure data deposition date)
AuthorsSchulz, L. / Zarzycki, J. / Steinchen, W. / Hochberg, G.K.A. / Erb, T.J.
External linksEmbo J. / PubMed:39558108
MethodsX-ray diffraction
Resolution1.75 - 1.85 Å
Structure data

PDB-8qmv:
L2 forming RubisCO derived from ancestral sequence reconstruction of the last common ancestor of Form I'' and Form I RubisCOs
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-8qmw:
Non-obligately L8S8-complex forming RubisCO derived from ancestral sequence reconstruction and rational engineering in L8S8 complex with substitutions R269W, E271R, L273N
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

Chemicals

ChemComp-CAP:
2-CARBOXYARABINITOL-1,5-DIPHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-B3P:
2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

Source
  • synthetic construct (others)
KeywordsLYASE / RubisCO / CABP / ancestral

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