EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Architecture and activation of single-pass transmembrane receptor guanylyl cyclase.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 32, Issue 3, Page 469-478, Year 2025
掲載日	2024年11月14日
著者	Shian Liu / Alexander M Payne / Jinan Wang / Lan Zhu / Navid Paknejad / Edward T Eng / Wei Liu / Yinglong Miao / Richard K Hite / Xin-Yun Huang /
PubMed 要旨	The heart, in addition to its primary role in blood circulation, functions as an endocrine organ by producing cardiac hormone natriuretic peptides. These hormones regulate blood pressure through the ...The heart, in addition to its primary role in blood circulation, functions as an endocrine organ by producing cardiac hormone natriuretic peptides. These hormones regulate blood pressure through the single-pass transmembrane receptor guanylyl cyclase A (GC-A), also known as natriuretic peptide receptor 1. The binding of the peptide hormones to the extracellular domain of the receptor activates the intracellular guanylyl cyclase domain of the receptor to produce the second messenger cyclic guanosine monophosphate. Despite their importance, the detailed architecture and domain interactions within full-length GC-A remain elusive. Here we present cryo-electron microscopy structures, functional analyses and molecular dynamics simulations of full-length human GC-A, in both the absence and the presence of atrial natriuretic peptide. The data reveal the architecture of full-length GC-A, highlighting the spatial arrangement of its various functional domains. This insight is crucial for understanding how different parts of the receptor interact and coordinate during activation. The study elucidates the molecular basis of how extracellular signals are transduced across the membrane to activate the intracellular guanylyl cyclase domain.
リンク	Nat Struct Mol Biol / PubMed:39543315
手法	EM (単粒子)
解像度	2.9 - 7.82 Å
構造データ	44429 9bcl EMDB-44429, PDB-9bcl: Extracellular domain of apo GC-A, state 1 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-44430: Full-length apo GC-A 手法: EM (単粒子) / 解像度: 7.82 Å 44431 9bcn EMDB-44431, PDB-9bcn: Extracellular domain of apo GC-A, state 2 手法: EM (単粒子) / 解像度: 2.9 Å 44432 9bco EMDB-44432, PDB-9bco: Intracellular domain of apo GC-A 手法: EM (単粒子) / 解像度: 4.4 Å 44433 9bcp EMDB-44433, PDB-9bcp: Kinase homology domain of apo GC-A 手法: EM (単粒子) / 解像度: 4.1 Å 44434 9bcq EMDB-44434, PDB-9bcq: Extracellular domain of GC-A bound to ANP 手法: EM (単粒子) / 解像度: 3.1 Å 44436 9bcs EMDB-44436, PDB-9bcs: Intracellular domain of GC-A bound to ANP 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-44437: Full-length GC-A bound to ANP 手法: EM (単粒子) / 解像度: 7.4 Å 44440 9bcv EMDB-44440, PDB-9bcv: Cyclase domain of GC-A bound to ANP 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-CL: Unknown entry / クロリド ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-HOH: WATER ChemComp-G2P: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMPCPP / GMP-CPP, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	homo sapiens (ヒト) mus musculus (ハツカネズミ)
キーワード	LYASE / Single pass transmembrane protein / guanylyl cyclase / atrial natriuretic peptide receptor / hypertension / membrane protein