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TitleNeIle, a Genetically Encoded Indicator for Branched-Chain Amino Acids Based on mNeonGreen Fluorescent Protein and LIVBP Protein.
Journal, issue, pagesACS Sens, Vol. 9, Page 5135-5147, Year 2024
Publish dateApr 9, 2024 (structure data deposition date)
AuthorsAsanova, A.N. / Subach, O.M. / Myachina, S.A. / Evteeva, M.A. / Gunitseva, N.M. / Borisova, A.A. / Patrushev, M.V. / Vlaskina, A.V. / Nikolaeva, A.Y. / Yang, L. ...Asanova, A.N. / Subach, O.M. / Myachina, S.A. / Evteeva, M.A. / Gunitseva, N.M. / Borisova, A.A. / Patrushev, M.V. / Vlaskina, A.V. / Nikolaeva, A.Y. / Yang, L. / Gabdulkhakov, A. / Dronova, E. / Samygina, V.R. / Xiao, X. / Zhao, H. / Piatkevich, K.D. / Subach, F.V.
External linksACS Sens / PubMed:39400357
MethodsX-ray diffraction
Resolution1.62 - 2.65 Å
Structure data

PDB-8z0g:
Crystal structure of NeIle complexed with isoleucine
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

PDB-9jti:
X-ray structure of NeIle indicator complexed with isoleucine
Method: X-RAY DIFFRACTION / Resolution: 1.62 Å

Chemicals

ChemComp-ILE:
ISOLEUCINE

ChemComp-GOL:
GLYCEROL

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

Source
  • escherichia coli o157:h7 (bacteria)
  • branchiostoma lanceolatum (amphioxus)
  • escherichia coli (E. coli)
KeywordsFLUORESCENT PROTEIN / fluorescent protein based sensor / chimeric protein / branched-chain amino acids indicator

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