EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of respiratory complex adaptation to cold temperatures.
ジャーナル・号・ページ	Cell, Vol. 187, Issue 23, Page 6584-6598.e17, Year 2024
掲載日	2024年11月14日
著者	Young-Cheul Shin / Pedro Latorre-Muro / Amina Djurabekova / Oleksii Zdorevskyi / Christopher F Bennett / Nils Burger / Kangkang Song / Chen Xu / Joao A Paulo / Steven P Gygi / Vivek Sharma / Maofu Liao / Pere Puigserver /
PubMed 要旨	In response to cold, mammals activate brown fat for respiratory-dependent thermogenesis reliant on the electron transport chain. Yet, the structural basis of respiratory complex adaptation upon cold ...In response to cold, mammals activate brown fat for respiratory-dependent thermogenesis reliant on the electron transport chain. Yet, the structural basis of respiratory complex adaptation upon cold exposure remains elusive. Herein, we combined thermoregulatory physiology and cryoelectron microscopy (cryo-EM) to study endogenous respiratory supercomplexes from mice exposed to different temperatures. A cold-induced conformation of CI:III (termed type 2) supercomplex was identified with a ∼25° rotation of CIII around its inter-dimer axis, shortening inter-complex Q exchange space, and exhibiting catalytic states that favor electron transfer. Large-scale supercomplex simulations in mitochondrial membranes reveal how lipid-protein arrangements stabilize type 2 complexes to enhance catalytic activity. Together, our cryo-EM studies, multiscale simulations, and biochemical analyses unveil the thermoregulatory mechanisms and dynamics of increased respiratory capacity in brown fat at the structural and energetic level.
リンク	Cell / PubMed:39395414 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 6.3 Å
構造データ	35313 8iao EMDB-35313, PDB-8iao: Respiratory complex CI:CIII2, type I, Wild type mouse under thermoneutral temperature 手法: EM (単粒子) / 解像度: 4.2 Å 35314 8iap EMDB-35314, PDB-8iap: Respiratory complex Peripheral Arm of CI, focus-refined map of type I, Wild type mouse under thermoneutral temperature 手法: EM (単粒子) / 解像度: 3.2 Å 35315 8iaq EMDB-35315, PDB-8iaq: Respiratory complex Membrane domain of CI, focused map of type I, Wild type mouse under thermoneutral temperature 手法: EM (単粒子) / 解像度: 3.4 Å 35316 8iar EMDB-35316, PDB-8iar: Respiratory complex CIII2, focus-refined of type I, Wild type mouse under thermoneutral temperature 手法: EM (単粒子) / 解像度: 3.4 Å 35331 8ib4 EMDB-35331, PDB-8ib4: Respiratory complex CI:CIII2, type IA, Wild type mouse under cold temperature 手法: EM (単粒子) / 解像度: 4.3 Å 35332 8ib5 EMDB-35332, PDB-8ib5: Respiratory complex Peripheral Arm of CI, focus-refined map of type IA, Wild type mouse under cold temperature 手法: EM (単粒子) / 解像度: 3.3 Å 35333 8ib6 EMDB-35333, PDB-8ib6: Respiratory complex Membrane domain of CI, focus-refined of type IA, Wild type mouse under cold temperature 手法: EM (単粒子) / 解像度: 3.3 Å 35334 8ib7 EMDB-35334, PDB-8ib7: Respiratory complex CIII2, focus-refined of type IA, Wild type mouse under cold temperature 手法: EM (単粒子) / 解像度: 3.4 Å 35336 8ib9 EMDB-35336, PDB-8ib9: Respiratory complex CI:CIII2, type IB, Wild type mouse under cold temperature 手法: EM (単粒子) / 解像度: 4.3 Å 35337 8iba EMDB-35337, PDB-8iba: Respiratory complex Peripheral Arm of CI, focus-refined map of type IB, Wild type mouse under cold temperature 手法: EM (単粒子) / 解像度: 3.2 Å 35338 8ibb EMDB-35338, PDB-8ibb: Respiratory complex Membrane domain of CI, focus-refined of type IB, Wild type mouse under cold temperature 手法: EM (単粒子) / 解像度: 3.3 Å 35339 8ibc EMDB-35339, PDB-8ibc: Respiratory complex CIII2, focus-refined of type IB, Wild type mouse under cold temperature 手法: EM (単粒子) / 解像度: 3.6 Å 35340 8ibd EMDB-35340, PDB-8ibd: Respiratory complex CI:CIII2, type II, Wild type mouse under cold temperature 手法: EM (単粒子) / 解像度: 4.2 Å 35341 8ibe EMDB-35341, PDB-8ibe: Respiratory complex Peripheral Arm of CI, focus-refined map of type II, Wild type mouse under cold temperature 手法: EM (単粒子) / 解像度: 3.3 Å 35342 8ibf EMDB-35342, PDB-8ibf: Respiratory complex Membrane domain of CI, focus-refined of type II, Wild type mouse under cold temperature 手法: EM (単粒子) / 解像度: 3.3 Å 35343 8ibg EMDB-35343, PDB-8ibg: Respiratory complex CIII2, focus-refined of type II, Wild type mouse under cold temperature 手法: EM (単粒子) / 解像度: 3.8 Å 35352 8ic2 EMDB-35352, PDB-8ic2: Respiratory complex CI:CIII2, type I, PERK -/- mouse under cold temperature 手法: EM (単粒子) / 解像度: 6.3 Å 35353 8ic3 EMDB-35353, PDB-8ic3: Respiratory complex Peripheral Arm of CI, focus-refined map of type I, PERK -/- mouse under cold temperature 手法: EM (単粒子) / 解像度: 3.2 Å 35354 8ic4 EMDB-35354, PDB-8ic4: Respiratory complex Membrane domain of CI, focus-refined of type I, PERK -/- mouse under cold temperature 手法: EM (単粒子) / 解像度: 3.2 Å 35355 8ic5 EMDB-35355, PDB-8ic5: Respiratory complex CIII2, focus-refined of type I, PERK -/- mouse under cold temperature 手法: EM (単粒子) / 解像度: 4.1 Å 38506 8xnl EMDB-38506, PDB-8xnl: Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature 手法: EM (単粒子) / 解像度: 3.1 Å 38507 8xnm EMDB-38507, PDB-8xnm: Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, Wild type mouse under thermoneutral temperature 手法: EM (単粒子) / 解像度: 3.5 Å 38508 8xnn EMDB-38508, PDB-8xnn: Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type I, Wild type mouse under thermoneutral temperature 手法: EM (単粒子) / 解像度: 3.6 Å 38509 8xno EMDB-38509, PDB-8xno: Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 3.4 Å 38510 8xnp EMDB-38510, PDB-8xnp: Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IA, Wild type mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 3.5 Å 38511 8xnq EMDB-38511, PDB-8xnq: Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IA, Wild type mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 3.7 Å 38512 8xnr EMDB-38512, PDB-8xnr: Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 3.3 Å 38513 8xns EMDB-38513, PDB-8xns: Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type IB, Wild type mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 3.5 Å 38514 8xnt EMDB-38514, PDB-8xnt: Respiratory complex Peripheral Arm of CI, close form C, focus-refined map of type IB, Wild type mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 4.1 Å 38515 8xnu EMDB-38515, PDB-8xnu: Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type IB, Wild type mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 3.6 Å 38516 8xnv EMDB-38516, PDB-8xnv: Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type II, Wild type mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 3.3 Å 38517 8xnw EMDB-38517, PDB-8xnw: Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type II, Wild type mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 3.6 Å 38518 8xnx EMDB-38518, PDB-8xnx: Respiratory complex Peripheral Arm of CI, open form A, focus-refined map of type II, Wild type mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 3.5 Å 38519 8xny EMDB-38519, PDB-8xny: Respiratory complex Peripheral Arm of CI, open form B, focus-refined map of type II, Wild type mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 4.1 Å 38520 8xnz EMDB-38520, PDB-8xnz: Respiratory complex Peripheral Arm of CI, close form A, focus-refined map of type I, PERK -/- mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 3.3 Å 38521 8xo0 EMDB-38521, PDB-8xo0: Respiratory complex Peripheral Arm of CI, close form B, focus-refined map of type I, PERK -/- mouse under Cold Acclimation 手法: EM (単粒子) / 解像度: 4.2 Å
化合物	ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / リン脂質YM ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-UQ1: UBIQUINONE-1 ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-FMN: FLAVIN MONONUCLEOTIDE ChemComp-UQ9: Ubiquinone-9 ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, エネルギー貯蔵分子YM ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE ChemComp-ZN: Unknown entry ChemComp-EHZ: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-U10: UBIQUINONE-10 ChemComp-UQ6: 5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL ChemComp-HEC: HEME C ChemComp-3PH: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE
由来	mus musculus (ハツカネズミ)
キーワード	ELECTRON TRANSPORT / Respiratory complex / Respiratory supercomplex