EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mycobacterial HelD connects RNA polymerase recycling with transcription initiation.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 8740, Year 2024
掲載日	2024年10月9日
著者	Tomáš Kovaľ / Nabajyoti Borah / Petra Sudzinová / Barbora Brezovská / Hana Šanderová / Viola Vaňková Hausnerová / Alena Křenková / Martin Hubálek / Mária Trundová / Kristýna Adámková / Jarmila Dušková / Marek Schwarz / Jana Wiedermannová / Jan Dohnálek / Libor Krásný / Tomáš Kouba /
PubMed 要旨	Mycobacterial HelD is a transcription factor that recycles stalled RNAP by dissociating it from nucleic acids and, if present, from the antibiotic rifampicin. The rescued RNAP, however, must ...Mycobacterial HelD is a transcription factor that recycles stalled RNAP by dissociating it from nucleic acids and, if present, from the antibiotic rifampicin. The rescued RNAP, however, must disengage from HelD to participate in subsequent rounds of transcription. The mechanism of release is unknown. We show that HelD from Mycobacterium smegmatis forms a complex with RNAP associated with the primary sigma factor σ and transcription factor RbpA but not CarD. We solve several structures of RNAP-σ-RbpA-HelD without and with promoter DNA. These snapshots capture HelD during transcription initiation, describing mechanistic aspects of HelD release from RNAP and its protective effect against rifampicin. Biochemical evidence supports these findings, defines the role of ATP binding and hydrolysis by HelD in the process, and confirms the rifampicin-protective effect of HelD. Collectively, these results show that when HelD is present during transcription initiation, the process is protected from rifampicin until the last possible moment.
リンク	Nat Commun / PubMed:39384756 / PubMed Central
手法	EM (単粒子)
解像度	3.09 - 3.89 Å
構造データ	18128 8q3i EMDB-18128, PDB-8q3i: Mycobacterium smegmatis RNA polymerase in complex with HelD, SigA and RbpA in State I 手法: EM (単粒子) / 解像度: 3.11 Å 18511 8qn8 EMDB-18511, PDB-8qn8: Mycobacterium smegmatis RNA polymerase in complex with HelD, SigA and RbpA in State II 手法: EM (単粒子) / 解像度: 3.14 Å 18650 8qti EMDB-18650, PDB-8qti: Mycobacterium smegnatis RNAP open promoter complex with SigmaA and RbpA 手法: EM (単粒子) / 解像度: 3.09 Å 18656 8qu6 EMDB-18656, PDB-8qu6: Mycobacterium smegnatis RNA polymerase transcription initiation complex with SigmaA, RbpA, HelD and an upstream-fork promoter fragment 手法: EM (単粒子) / 解像度: 3.45 Å 18851 8r2m EMDB-18851, PDB-8r2m: Mycobacterium smegnatis RNA polymerase transcription initiation complex with SigmaA, RbpA, HelD N-terminal domain and an upstream-fork promoter fragment; State III conformation 手法: EM (単粒子) / 解像度: 3.44 Å 18873 8r3m EMDB-18873, PDB-8r3m: Mycobacterium smegnatis RNA polymerase transcription initiation complex with SigmaA, RbpA, HelD N-terminal, CO and PCh loop domain, and an upstream-fork promoter fragment; State III conformation 手法: EM (単粒子) / 解像度: 3.49 Å 18956 8r6p EMDB-18956, PDB-8r6p: Mycobacterium smegnatis RNA polymerase RP2-like transcription initiation complex with SigmaA, RbpA, HelD N-terminal domain and open promoter DNA 手法: EM (単粒子) / 解像度: 3.16 Å 18959 8r6r EMDB-18959, PDB-8r6r: Mycobacterium smegnatis RNA polymerase RP2-like transcription initiation complex with SigmaA, RbpA and open promoter DNA 手法: EM (単粒子) / 解像度: 3.89 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	mycolicibacterium smegmatis mc2 155 (バクテリア)
キーワード	TRANSCRIPTION / HelD / RNA polymerase / transcription initiation / Mycobacteria / rifampicin resistance