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-Structure paper
タイトル | Structure of Fanzor2 reveals insights into the evolution of the TnpB superfamily. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Year 2024 |
掲載日 | 2024年10月1日 |
著者 | Richard D Schargel / M Zuhaib Qayyum / Ajay Singh Tanwar / Ravi C Kalathur / Elizabeth H Kellogg / |
PubMed 要旨 | RNA-guided endonucleases, once thought to be exclusive to prokaryotes, have been recently identified in eukaryotes and are called Fanzors. They are classified into two clades, Fanzor1 and Fanzor2. ...RNA-guided endonucleases, once thought to be exclusive to prokaryotes, have been recently identified in eukaryotes and are called Fanzors. They are classified into two clades, Fanzor1 and Fanzor2. Here we present the cryo-electron microscopy structure of Acanthamoeba polyphaga mimivirus Fanzor2, revealing its ωRNA architecture, active site and features involved in transposon-adjacent motif recognition. A comparison to Fanzor1 and TnpB structures highlights divergent evolutionary paths, advancing our understanding of RNA-guided endonucleases. |
リンク | Nat Struct Mol Biol / PubMed:39354233 |
手法 | EM (単粒子) |
解像度 | 2.99 Å |
構造データ | EMDB-44046, PDB-9b0l: |
化合物 | ChemComp-ZN: ChemComp-MG: |
由来 |
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キーワード | DNA BINDING PROTEIN/DNA/RNA / Transposon / Fanzor / cryo-EM / DNA BINDING PROTEIN-DNA-RNA complex |