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TitleMechanism of enterovirus VP0 maturation cleavage based on the structure of a stabilised assembly intermediate.
Journal, issue, pagesPLoS Pathog, Vol. 20, Issue 9, Page e1012511, Year 2024
Publish dateSep 19, 2024
AuthorsNatalie J Kingston / Joseph S Snowden / Keith Grehan / Philippa K Hall / Eero V Hietanen / Tim C Passchier / Stephen J Polyak / David J Filman / James M Hogle / David J Rowlands / Nicola J Stonehouse /
PubMed AbstractMolecular details of genome packaging are little understood for the majority of viruses. In enteroviruses (EVs), cleavage of the structural protein VP0 into VP4 and VP2 is initiated by the ...Molecular details of genome packaging are little understood for the majority of viruses. In enteroviruses (EVs), cleavage of the structural protein VP0 into VP4 and VP2 is initiated by the incorporation of RNA into the assembling virion and is essential for infectivity. We have applied a combination of bioinformatic, molecular and structural approaches to generate the first high-resolution structure of an intermediate in the assembly pathway, termed a provirion, which contains RNA and intact VP0. We have demonstrated an essential role of VP0 E096 in VP0 cleavage independent of RNA encapsidation and generated a new model of capsid maturation, supported by bioinformatic analysis. This provides a molecular basis for RNA-dependence, where RNA induces conformational changes required for VP0 maturation, but that RNA packaging itself is not sufficient to induce maturation. These data have implications for understanding production of infectious virions and potential relevance for future vaccine and antiviral drug design.
External linksPLoS Pathog / PubMed:39298524 / PubMed Central
MethodsEM (single particle)
Resolution2.5 - 2.7 Å
Structure data

EMDB-50200: EVA71 E096A native particle
Method: EM (single particle) / Resolution: 2.5 Å

EMDB-50221: EVA71 E096A native particle
Method: EM (single particle) / Resolution: 2.7 Å

Source
  • Enterovirus A71

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