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-Structure paper
| タイトル | Cryo-EM-based discovery of a pathogenic parvovirus causing epidemic mortality by black wasting disease in farmed beetles. |
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| ジャーナル・号・ページ | Cell, Vol. 187, Issue 20, Page 5604-5619.e14, Year 2024 |
| 掲載日 | 2024年10月3日 |
著者 | Judit J Penzes / Martin Holm / Samantha A Yost / Jason T Kaelber / ![]() |
| PubMed 要旨 | We use cryoelectron microscopy (cryo-EM) as a sequence- and culture-independent diagnostic tool to identify the etiological agent of an agricultural pandemic. For the past 4 years, American insect- ...We use cryoelectron microscopy (cryo-EM) as a sequence- and culture-independent diagnostic tool to identify the etiological agent of an agricultural pandemic. For the past 4 years, American insect-rearing facilities have experienced a distinctive larval pathology and colony collapse of farmed Zophobas morio (superworm). By means of cryo-EM, we discovered the causative agent: a densovirus that we named Zophobas morio black wasting virus (ZmBWV). We confirmed the etiology of disease by fulfilling Koch's postulates and characterizing strains from across the United States. ZmBWV is a member of the family Parvoviridae with a 5,542 nt genome, and we describe intersubunit interactions explaining its expanded internal volume relative to human parvoviruses. Cryo-EM structures at resolutions up to 2.1 Å revealed single-strand DNA (ssDNA) ordering at the capsid inner surface pinned by base-binding pockets in the capsid inner surface. Also, we demonstrated the prophylactic potential of non-pathogenic strains to provide cross-protection in vivo. |
リンク | Cell / PubMed:39208798 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.0 - 3.4 Å |
| 構造データ | EMDB-41106, PDB-8t9c: EMDB-41108, PDB-8t9e: EMDB-41125, PDB-8t9x: EMDB-41131, PDB-8ta7: EMDB-41301, PDB-8tje: |
| 化合物 | ![]() ChemComp-HOH: |
| 由来 |
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キーワード | VIRUS/DNA / Capsid / Virion / Parvovirus / Densovirus / Invertebrate / Insect / Pathogen / ssDNA / VIRUS / VIRUS-DNA complex |
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zophobas morio (甲虫)
zophobas morio black wasting virus (ウイルス)
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