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Structure paper

TitleStructural insights into the chloroplast protein import in land plants.
Journal, issue, pagesCell, Vol. 187, Issue 20, Page 5651-5664.e18, Year 2024
Publish dateOct 3, 2024
AuthorsKe Liang / Zeyu Jin / Xiechao Zhan / Yuxin Li / Qikui Xu / Yanqiu Xie / Yi Yang / Shaojie Wang / Jianping Wu / Zhen Yan /
PubMed AbstractChloroplast proteins are imported via the translocon at the outer chloroplast membrane (TOC)-translocon at the inner chloroplast membrane (TIC) supercomplex, driven by an ATPase motor. The Ycf2-FtsHi ...Chloroplast proteins are imported via the translocon at the outer chloroplast membrane (TOC)-translocon at the inner chloroplast membrane (TIC) supercomplex, driven by an ATPase motor. The Ycf2-FtsHi complex has been identified as the chloroplast import motor. However, its assembly and cooperation with the TIC complex during preprotein translocation remain unclear. Here, we present the structures of the Ycf2-FtsHi and TIC complexes from Arabidopsis and an ultracomplex formed between them from Pisum. The Ycf2-FtsHi structure reveals a heterohexameric AAA+ ATPase motor module with characteristic features. Four previously uncharacterized components of Ycf2-FtsHi were identified, which aid in complex assembly and anchoring of the motor module at a tilted angle relative to the membrane. When considering the structures of the TIC complex and the TIC-Ycf2-FtsHi ultracomplex together, it becomes evident that the tilted motor module of Ycf2-FtsHi enables its close contact with the TIC complex, thereby facilitating efficient preprotein translocation. Our study provides valuable structural insights into the chloroplast protein import process in land plants.
External linksCell / PubMed:39197452
MethodsEM (single particle)
Resolution2.5 - 11.0 Å
Structure data

38425

8xku

EMDB-38425: The composite EM map of the Ycf2-FtsHi motor complex from Arabidopsis in ATP-bound state
PDB-8xku: Cryo-EM structure of the Ycf2-FtsHi motor complex from Arabidopsis in ATP-bound state
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-38426: Overall map of the Ycf2-FtsHi motor complex from Arabidopsis in ATP-bound state
Method: EM (single particle) / Resolution: 3.2 Å

EMDB-38427: Local EM map of the AtYcf2-FtsHi motor complex in the inter-membrane space region (ATP dataset)
Method: EM (single particle) / Resolution: 3.8 Å

38428

8xkv

EMDB-38428, PDB-8xkv:
Cryo-EM structure of the Ycf2-FtsHi motor complex from Arabidopsis in Apo state
Method: EM (single particle) / Resolution: 3.3 Å

39872

8z9y

EMDB-39872, PDB-8z9y:
Cryo-EM Structure of the Arabidopsis thaliana TIC Complex
Method: EM (single particle) / Resolution: 2.5 Å

EMDB-39948: Cryo-EM Map of Pisum sativum TOC-TIC-Ycf2-FtsHi Ultracomplex
Method: EM (single particle) / Resolution: 11.0 Å

EMDB-60068: Local EM map of the AtYcf2-FtsHi motor complex in the inter-membrane space region (Apo dataset)
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-60069: Composite map of the Ycf2-FtsHi motor complex from Arabidopsis in apo state
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-ZN:
Unknown entry

ChemComp-PX2:
1,2-DILAUROYL-SN-GLYCERO-3-PHOSPHATE

Source
  • arabidopsis thaliana (thale cress)
  • Pisum sativum (garden pea)
KeywordsMEMBRANE PROTEIN / ATP-motor / Ycf2 / FtsHi / TRANSLOCASE / Complex / Channel / Membrane protein.

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