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TitleAn image processing pipeline for electron cryo-tomography in RELION-5.
Journal, issue, pagesFEBS Open Bio, Vol. 14, Issue 11, Page 1788-1804, Year 2024
Publish dateAug 15, 2024
AuthorsAlister Burt / Bogdan Toader / Rangana Warshamanage / Andriko von Kügelgen / Euan Pyle / Jasenko Zivanov / Dari Kimanius / Tanmay A M Bharat / Sjors H W Scheres /
PubMed AbstractElectron tomography of frozen, hydrated samples allows structure determination of macromolecular complexes that are embedded in complex environments. Provided that the target complexes may be ...Electron tomography of frozen, hydrated samples allows structure determination of macromolecular complexes that are embedded in complex environments. Provided that the target complexes may be localised in noisy, three-dimensional tomographic reconstructions, averaging images of multiple instances of these molecules can lead to structures with sufficient resolution for de novo atomic modelling. Although many research groups have contributed image processing tools for these tasks, a lack of standardisation and interoperability represents a barrier for newcomers to the field. Here, we present an image processing pipeline for electron tomography data in RELION-5, with functionality ranging from the import of unprocessed movies to the automated building of atomic models in the final maps. Our explicit definition of metadata items that describe the steps of our pipeline has been designed for interoperability with other software tools and provides a framework for further standardisation.
External linksFEBS Open Bio / PubMed:39147729 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution3.3 Å
Structure data

EMDB-50672: A 3.3A sub-tomogram average of HIV-1 CA-SP1 from 5 tomograms in EMPIAR-10164 obtained using RELION 5
Method: EM (subtomogram averaging) / Resolution: 3.3 Å

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