EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for the activity of the type VII CRISPR-Cas system.
ジャーナル・号・ページ	Nature, Vol. 633, Issue 8029, Page 465-472, Year 2024
掲載日	2024年8月14日
著者	Jie Yang / Xuzichao Li / Qiuqiu He / Xiaoshen Wang / Jingjing Tang / Tongyao Wang / Yi Zhang / Feiyang Yu / Shuqin Zhang / Zhikun Liu / Lingling Zhang / Fumeng Liao / Hang Yin / Haiyan Zhao / Zengqin Deng / Heng Zhang /
PubMed 要旨	The newly identified type VII CRISPR-Cas candidate system uses a CRISPR RNA-guided ribonucleoprotein complex formed by Cas5 and Cas7 proteins to target RNA. However, the RNA cleavage is executed by a ...The newly identified type VII CRISPR-Cas candidate system uses a CRISPR RNA-guided ribonucleoprotein complex formed by Cas5 and Cas7 proteins to target RNA. However, the RNA cleavage is executed by a dedicated Cas14 nuclease, which is distinct from the effector nucleases of the other CRISPR-Cas systems. Here we report seven cryo-electron microscopy structures of the Cas14-bound interference complex at different functional states. Cas14, a tetrameric protein in solution, is recruited to the Cas5-Cas7 complex in a target RNA-dependent manner. The N-terminal catalytic domain of Cas14 binds a stretch of the substrate RNA for cleavage, whereas the C-terminal domain is primarily responsible for tethering Cas14 to the Cas5-Cas7 complex. The biochemical cleavage assays corroborate the captured functional conformations, revealing that Cas14 binds to different sites on the Cas5-Cas7 complex to execute individual cleavage events. Notably, a plugged-in arginine of Cas7 sandwiched by a C-shaped clamp of C-terminal domain precisely modulates Cas14 binding. More interestingly, target RNA cleavage is altered by a complementary protospacer flanking sequence at the 5' end, but not at the 3' end. Altogether, our study elucidates critical molecular details underlying the assembly of the interference complex and substrate cleavage in the type VII CRISPR-Cas system, which may help rational engineering of the type VII CRISPR-Cas system for biotechnological applications.
リンク	Nature / PubMed:39143216
手法	EM (単粒子)
解像度	2.85 - 3.2 Å
構造データ	39287 8yhd EMDB-39287, PDB-8yhd: Cryo-EM structure of CTR-bound type VII CRISPR-Cas complex at post-state I 手法: EM (単粒子) / 解像度: 2.93 Å 39288 8yhe EMDB-39288, PDB-8yhe: Cryo-EM structure of CTR-bound type VII CRISPR-Cas complex at post-state II 手法: EM (単粒子) / 解像度: 3.07 Å 39766 8z4j EMDB-39766, PDB-8z4j: Cryo-EM structure of CTR-bound type VII CRISPR-Cas complex at substrate-engaged state II 手法: EM (単粒子) / 解像度: 2.97 Å 39767 8z4l EMDB-39767, PDB-8z4l: Cryo-EM structure of CTR-bound type VII CRISPR-Cas complex at substrate-engaged state I 手法: EM (単粒子) / 解像度: 2.85 Å 39857 8z99 EMDB-39857, PDB-8z99: Cryo-EM structure of NTR-bound type VII CRISPR-Cas complex at substrate-engaged state +I 手法: EM (単粒子) / 解像度: 3.2 Å 39859 8z9c EMDB-39859, PDB-8z9c: Cryo-EM structure of NTR-bound type VII CRISPR-Cas complex at substrate-engaged state I 手法: EM (単粒子) / 解像度: 3.01 Å 39861 8z9e EMDB-39861, PDB-8z9e: Cryo-EM structure of NTR-bound type VII CRISPR-Cas complex at substrate-engaged state II 手法: EM (単粒子) / 解像度: 3.13 Å
化合物	ChemComp-ZN: Unknown entry
由来	metagenome (メタゲノム)
キーワード	ANTIVIRAL PROTEIN / a protein complex / protein structure