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Structure paper

TitleStructural Insights into the Catalytic Cycle of Inulin Fructotransferase: From Substrate Anchoring to Product Releasing.
Journal, issue, pagesJ. Agric. Food Chem., Vol. 72, Page 17030-17040, Year 2024
Publish dateDec 20, 2022 (structure data deposition date)
AuthorsCheng, M. / Hou, X. / Huang, Z. / Chen, Z. / Ni, D. / Zhang, W. / Rao, Y. / Mu, W.
External linksJ. Agric. Food Chem. / PubMed:39034843
MethodsX-ray diffraction
Resolution1.44 - 1.69 Å
Structure data

PDB-8hsn:
Crystal structure of DFA I-forming Inulin Lyase from Streptomyces peucetius subsp. caesius ATCC 27952
Method: X-RAY DIFFRACTION / Resolution: 1.69 Å

PDB-8hui:
Crystal structure of DFA I-forming Inulin Lyase from Streptomyces peucetius subsp. caesius ATCC 27952 in complex with GF4, DFA I, and fructose
Method: X-RAY DIFFRACTION / Resolution: 1.44 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-2PO:
PHOSPHONATE

ChemComp-HOH:
WATER

ChemComp-FRU:
beta-D-fructofuranose

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

Source
  • streptomyces peucetius subsp. caesius atcc 27952 (bacteria)
KeywordsLYASE / right-handed beta-helix protein / DFA I-forming inulin lyase / complex

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