+検索条件
-Structure paper
タイトル | Implicating the red body of Nannochloropsis in forming the recalcitrant cell wall polymer algaenan. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 5456, Year 2024 |
掲載日 | 2024年6月27日 |
著者 | Christopher W Gee / Johan Andersen-Ranberg / Ethan Boynton / Rachel Z Rosen / Danielle Jorgens / Patricia Grob / Hoi-Ying N Holman / Krishna K Niyogi / |
PubMed 要旨 | Stramenopile algae contribute significantly to global primary productivity, and one class, Eustigmatophyceae, is increasingly studied for applications in high-value lipid production. Yet much about ...Stramenopile algae contribute significantly to global primary productivity, and one class, Eustigmatophyceae, is increasingly studied for applications in high-value lipid production. Yet much about their basic biology remains unknown, including the nature of an enigmatic, pigmented globule found in vegetative cells. Here, we present an in-depth examination of this "red body," focusing on Nannochloropsis oceanica. During the cell cycle, the red body forms adjacent to the plastid, but unexpectedly it is secreted and released with the autosporangial wall following cell division. Shed red bodies contain antioxidant ketocarotenoids, and overexpression of a beta-carotene ketolase results in enlarged red bodies. Infrared spectroscopy indicates long-chain, aliphatic lipids in shed red bodies and cell walls, and UHPLC-HRMS detects a C32 alkyl diol, a potential precursor of algaenan, a recalcitrant cell wall polymer. We propose that the red body transports algaenan precursors from plastid to apoplast to be incorporated into daughter cell walls. |
リンク | Nat Commun / PubMed:38937455 / PubMed Central |
手法 | EM (トモグラフィー) |
構造データ | EMDB-43893: Structure of the auto-fluorescent membrane-bound red body organelle from Nannochloropsis oceanica in situ |
由来 |
|