EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of the mumps virus polymerase complex via cryo-electron microscopy.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 4189, Year 2024
掲載日	2024年5月17日
著者	Tianhao Li / Mingdong Liu / Zhanxi Gu / Xin Su / Yunhui Liu / Jinzhong Lin / Yu Zhang / Qing-Tao Shen /
PubMed 要旨	The viral polymerase complex, comprising the large protein (L) and phosphoprotein (P), is crucial for both genome replication and transcription in non-segmented negative-strand RNA viruses (nsNSVs), ...The viral polymerase complex, comprising the large protein (L) and phosphoprotein (P), is crucial for both genome replication and transcription in non-segmented negative-strand RNA viruses (nsNSVs), while structures corresponding to these activities remain obscure. Here, we resolved two L-P complex conformations from the mumps virus (MuV), a typical member of nsNSVs, via cryogenic-electron microscopy. One conformation presents all five domains of L forming a continuous RNA tunnel to the methyltransferase domain (MTase), preferably as a transcription state. The other conformation has the appendage averaged out, which is inaccessible to MTase. In both conformations, parallel P tetramers are revealed around MuV L, which, together with structures of other nsNSVs, demonstrates the diverse origins of the L-binding X domain of P. Our study links varying structures of nsNSV polymerase complexes with genome replication and transcription and points to a sliding model for polymerase complexes to advance along the RNA templates.
リンク	Nat Commun / PubMed:38760379 / PubMed Central
手法	EM (単粒子)
解像度	2.93 - 3.63 Å
構造データ	35864 8izl EMDB-35864: Structure of the Mumps Virus L Protein Bound by Phosphoprotein Tetramer (composite map) PDB-8izl: Structure of the Mumps Virus L Protein Bound by Phosphoprotein Tetramer 手法: EM (単粒子) / 解像度: 2.93 Å EMDB-37957: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer 手法: EM (単粒子) / 解像度: 3.02 Å 37958 8yxl EMDB-37958: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer (Focused map for CD-MTase-CTD) PDB-8yxl: Structure of C-terminal domain of L protein from Mumps virus 手法: EM (単粒子) / 解像度: 3.13 Å 37959 8yxm EMDB-37959: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer (Focused map for RdRp-PRNTase) PDB-8yxm: Structure of N-terminal domain of L protein bound with Phosphoprotein from Mumps Virus 手法: EM (単粒子) / 解像度: 2.93 Å 37960 8yxo EMDB-37960: Cryo-EM map for Mumps Virus L Protein Bound by Phosphoprotein Tetramer (Focused map for tetrameric phosphoproteins) PDB-8yxo: Structure of Phosphoprotein tetramer from mumps virus 手法: EM (単粒子) / 解像度: 3.49 Å 37961 8yxp EMDB-37961: Cryo-EM map for Mumps Virus L Protein (State 2) Bound by Phosphoprotein Tetramer PDB-8yxp: Structure of mumps virus L protein (state2) 手法: EM (単粒子) / 解像度: 3.01 Å 37962 8yxr EMDB-37962: Cryo-EM map for Mumps Virus L protein (state2) Bound by Phosphoprotein Tetramer (Focused for tetrameric phosphoprotein) PDB-8yxr: Structure of Phosphoprotein Tetramer from mumps virus 手法: EM (単粒子) / 解像度: 3.63 Å 37964 8x01 EMDB-37964: Structure of the Mumps Virus L Protein (state2) Bound by Phosphoprotein Tetramer (composite map) PDB-8x01: Structure of the Mumps Virus L Protein (state2) Bound by Phosphoprotein Tetramer 手法: EM (単粒子) / 解像度: 3.01 Å
化合物	ChemComp-ZN: Unknown entry
由来	mumps orthorubulavirus (ウイルス)
キーワード	VIRAL PROTEIN / Mumps virus polymerase complex / RNA-dependent RNA synthesis / Large protein / phosphoprotein.