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-Structure paper
タイトル | A structural and dynamic visualization of the interaction between MAP7 and microtubules. |
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ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 1948, Year 2024 |
掲載日 | 2024年3月2日 |
著者 | Agnes Adler / Mamata Bangera / J Wouter Beugelink / Salima Bahri / Hugo van Ingen / Carolyn A Moores / Marc Baldus / |
PubMed 要旨 | Microtubules (MTs) are key components of the eukaryotic cytoskeleton and are essential for intracellular organization, organelle trafficking and mitosis. MT tasks depend on binding and interactions ...Microtubules (MTs) are key components of the eukaryotic cytoskeleton and are essential for intracellular organization, organelle trafficking and mitosis. MT tasks depend on binding and interactions with MT-associated proteins (MAPs). MT-associated protein 7 (MAP7) has the unusual ability of both MT binding and activating kinesin-1-mediated cargo transport along MTs. Additionally, the protein is reported to stabilize MTs with its 112 amino-acid long MT-binding domain (MTBD). Here we investigate the structural basis of the interaction of MAP7 MTBD with the MT lattice. Using a combination of solid and solution-state nuclear magnetic resonance (NMR) spectroscopy with electron microscopy, fluorescence anisotropy and isothermal titration calorimetry, we shed light on the binding mode of MAP7 to MTs at an atomic level. Our results show that a combination of interactions between MAP7 and MT lattice extending beyond a single tubulin dimer and including tubulin C-terminal tails contribute to formation of the MAP7-MT complex. |
リンク | Nat Commun / PubMed:38431715 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.7 - 4.0 Å |
構造データ | EMDB-19042, PDB-8rc1: EMDB-19043: MAP7 MTBD (microtubule binding domain) decorated Taxol stabilized microtubule (13pf) protofilament EMDB-19044: MAP7 MTBD (microtubule binding domain) decorated Taxol stabilized microtubule (14pf) protofilament |
化合物 | ChemComp-GTP: ChemComp-MG: ChemComp-GDP: |
由来 |
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キーワード | STRUCTURAL PROTEIN (タンパク質) / Microtubule associated protein 7(MAP 7) / microtubule binding domain / cryo-EM (低温電子顕微鏡法) |