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-Structure paper
タイトル | A metabolically controlled contact site between vacuoles and lipid droplets in yeast. |
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ジャーナル・号・ページ | Dev Cell, Vol. 59, Issue 6, Page 740-758.e10, Year 2024 |
掲載日 | 2024年3月25日 |
著者 | Duy Trong Vien Diep / Javier Collado / Marie Hugenroth / Rebecca Martina Fausten / Louis Percifull / Mike Wälte / Christian Schuberth / Oliver Schmidt / Rubén Fernández-Busnadiego / Maria Bohnert / |
PubMed 要旨 | The lipid droplet (LD) organization proteins Ldo16 and Ldo45 affect multiple aspects of LD biology in yeast. They are linked to the LD biogenesis machinery seipin, and their loss causes defects in LD ...The lipid droplet (LD) organization proteins Ldo16 and Ldo45 affect multiple aspects of LD biology in yeast. They are linked to the LD biogenesis machinery seipin, and their loss causes defects in LD positioning, protein targeting, and breakdown. However, their molecular roles remained enigmatic. Here, we report that Ldo16/45 form a tether complex with Vac8 to create vacuole lipid droplet (vCLIP) contact sites, which can form in the absence of seipin. The phosphatidylinositol transfer protein (PITP) Pdr16 is a further vCLIP-resident recruited specifically by Ldo45. While only an LD subpopulation is engaged in vCLIPs at glucose-replete conditions, nutrient deprivation results in vCLIP expansion, and vCLIP defects impair lipophagy upon prolonged starvation. In summary, Ldo16/45 are multifunctional proteins that control the formation of a metabolically regulated contact site. Our studies suggest a link between LD biogenesis and breakdown and contribute to a deeper understanding of how lipid homeostasis is maintained during metabolic challenges. |
リンク | Dev Cell / PubMed:38367622 |
手法 | EM (トモグラフィー) |
構造データ | EMDB-18892: Lipid droplet-Vacuole contacts in Ldo16 overexpression yeast strain. EMDB-18893: Lipid droplet-vacuole and Nucleus-vacuole contacts in WT yeast cell starved for 4 hours EMDB-18894: Lipid droplet lipophagy in 4-hour starved WT yeast cell. EMDB-18895: Multiple vacuole-lipid droplet-nucleus contacts in 4-hour starved WT yeast cell. EMDB-18896: Lipophagy in 5-day starved WT yeast cell. EMDB-18897: Lipid droplets in proximity to a vacuole in dLdo strain cell after 5-day starvation. EMDB-18898: Vacuolar contents of WT cell after 5-day starvation. EMDB-18899: Lipid droplet-nucleus contacts in dLdo yeast strain after 5-day starvation. |
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