Gerty Cori Programme, Medical Faculty, University of Munster
EXC 1003 - CiM
Germany
German Research Foundation (DFG)
264061860
Germany
Germanys Excellence Strategy
EXC 2067/1-390729940
Germany
German Research Foundation (DFG)
448415290
Germany
Austrian Science Fund
P36187-B
Austria
Citation
Journal: Dev Cell / Year: 2024 Title: A metabolically controlled contact site between vacuoles and lipid droplets in yeast. Authors: Duy Trong Vien Diep / Javier Collado / Marie Hugenroth / Rebecca Martina Fausten / Louis Percifull / Mike Wälte / Christian Schuberth / Oliver Schmidt / Rubén Fernández-Busnadiego / Maria Bohnert / Abstract: The lipid droplet (LD) organization proteins Ldo16 and Ldo45 affect multiple aspects of LD biology in yeast. They are linked to the LD biogenesis machinery seipin, and their loss causes defects in LD ...The lipid droplet (LD) organization proteins Ldo16 and Ldo45 affect multiple aspects of LD biology in yeast. They are linked to the LD biogenesis machinery seipin, and their loss causes defects in LD positioning, protein targeting, and breakdown. However, their molecular roles remained enigmatic. Here, we report that Ldo16/45 form a tether complex with Vac8 to create vacuole lipid droplet (vCLIP) contact sites, which can form in the absence of seipin. The phosphatidylinositol transfer protein (PITP) Pdr16 is a further vCLIP-resident recruited specifically by Ldo45. While only an LD subpopulation is engaged in vCLIPs at glucose-replete conditions, nutrient deprivation results in vCLIP expansion, and vCLIP defects impair lipophagy upon prolonged starvation. In summary, Ldo16/45 are multifunctional proteins that control the formation of a metabolically regulated contact site. Our studies suggest a link between LD biogenesis and breakdown and contribute to a deeper understanding of how lipid homeostasis is maintained during metabolic challenges.
Cryogen name: ETHANE-PROPANE / Chamber humidity: 80 % / Chamber temperature: 297 K / Instrument: FEI VITROBOT MARK IV / Details: Back-blotted.
Details
Cells were plunge-frozen at 0.6 O.D600 concentration.
Sectioning
Focused ion beam - Instrument: OTHER / Focused ion beam - Ion: OTHER / Focused ion beam - Voltage: 30 / Focused ion beam - Current: 0.1 / Focused ion beam - Duration: 1000 / Focused ion beam - Temperature: 78 K / Focused ion beam - Initial thickness: 4000 / Focused ion beam - Final thickness: 200 Focused ion beam - Details: The value given for _em_focused_ion_beam.instrument is Thermo Fisher Aquilos 2 FIB. This is not in a list of allowed values {'DB235', 'OTHER'} so OTHER is written into the XML file.
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Electron microscopy
Microscope
FEI TITAN KRIOS
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
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