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-Structure paper
タイトル | Evolving precision: rRNA expansion segment 7S modulates translation velocity and accuracy in eukaryal ribosomes. |
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ジャーナル・号・ページ | Nucleic Acids Res, Vol. 52, Issue 7, Page 4021-4036, Year 2024 |
掲載日 | 2024年4月24日 |
![]() | Robert Rauscher / Cristian Eggers / Lyudmila Dimitrova-Paternoga / Vaishnavi Shankar / Alessia Rosina / Marina Cristodero / Helge Paternoga / Daniel N Wilson / Sebastian A Leidel / Norbert Polacek / ![]() ![]() |
PubMed 要旨 | Ribosome-enhanced translational miscoding of the genetic code causes protein dysfunction and loss of cellular fitness. During evolution, open reading frame length increased, necessitating mechanisms ...Ribosome-enhanced translational miscoding of the genetic code causes protein dysfunction and loss of cellular fitness. During evolution, open reading frame length increased, necessitating mechanisms for enhanced translation fidelity. Indeed, eukaryal ribosomes are more accurate than bacterial counterparts, despite their virtually identical, conserved active centers. During the evolution of eukaryotic organisms ribosome expansions at the rRNA and protein level occurred, which potentially increases the options for translation regulation and cotranslational events. Here we tested the hypothesis that ribosomal RNA expansions can modulate the core function of the ribosome, faithful protein synthesis. We demonstrate that a short expansion segment present in all eukaryotes' small subunit, ES7S, is crucial for accurate protein synthesis as its presence adjusts codon-specific velocities and guarantees high levels of cognate tRNA selection. Deletion of ES7S in yeast enhances mistranslation and causes protein destabilization and aggregation, dramatically reducing cellular fitness. Removal of ES7S did not alter ribosome architecture but altered the structural dynamics of inter-subunit bridges thus affecting A-tRNA selection. Exchanging the yeast ES7S sequence with the human ES7S increases accuracy whereas shortening causes the opposite effect. Our study demonstrates that ES7S provided eukaryal ribosomes with higher accuracy without perturbing the structurally conserved decoding center. |
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手法 | EM (単粒子) |
解像度 | 2.4 Å |
構造データ | EMDB-16127, PDB-8bn3: |
化合物 | ![]() ChemComp-MG: ![]() ChemComp-K: ![]() ChemComp-3HE: ![]() ChemComp-SPD: ![]() ChemComp-ZN: ![]() ChemComp-HOH: |
由来 |
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![]() | RIBOSOME / eIF5A / Stm1 / Rpl41A / delta ES7s |