+Search query
-Structure paper
Title | Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores. |
---|---|
Journal, issue, pages | Nat Struct Mol Biol, Vol. 31, Issue 7, Page 1114-1123, Year 2024 |
Publish date | Feb 5, 2024 |
Authors | Julia Datler / Jesse M Hansen / Andreas Thader / Alois Schlögl / Lukas W Bauer / Victor-Valentin Hodirnau / Florian K M Schur / |
PubMed Abstract | Poxviruses are among the largest double-stranded DNA viruses, with members such as variola virus, monkeypox virus and the vaccination strain vaccinia virus (VACV). Knowledge about the structural ...Poxviruses are among the largest double-stranded DNA viruses, with members such as variola virus, monkeypox virus and the vaccination strain vaccinia virus (VACV). Knowledge about the structural proteins that form the viral core has remained sparse. While major core proteins have been annotated via indirect experimental evidence, their structures have remained elusive and they could not be assigned to individual core features. Hence, which proteins constitute which layers of the core, such as the palisade layer and the inner core wall, has remained enigmatic. Here we show, using a multi-modal cryo-electron microscopy (cryo-EM) approach in combination with AlphaFold molecular modeling, that trimers formed by the cleavage product of VACV protein A10 are the key component of the palisade layer. This allows us to place previously obtained descriptions of protein interactions within the core wall into perspective and to provide a detailed model of poxvirus core architecture. Importantly, we show that interactions within A10 trimers are likely generalizable over members of orthopox- and parapoxviruses. |
External links | Nat Struct Mol Biol / PubMed:38316877 / PubMed Central |
Methods | EM (single particle) / EM (subtomogram averaging) / EM (tomography) |
Resolution | 3.8 - 20.7 Å |
Structure data | EMDB-17410, PDB-8p4k: EMDB-17411: Subtomogram averaging structure of the Vaccinia Virus core palisade layer EMDB-17412: Vaccinia Virus flower-shaped pore-like structure EMDB-17413: Cryo-electron tomogram of intact mature Vaccinia Virus particle EMDB-17414: Cryo-electron tomogram of isolated Vaccinia Virus cores EMDB-18452: Vaccinia virus inner core wall |
Source |
|
Keywords | VIRAL PROTEIN / Poxvirus / Vaccinia Virus / core / cryo-electron tomography / AlphaFold |