EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Product analog binding identifies the copper active site of particulate methane monooxygenase.
ジャーナル・号・ページ	Nat Catal, Vol. 6, Issue 12, Page 1194-1204, Year 2023
掲載日	2023年11月6日
著者	Frank J Tucci / Richard J Jodts / Brian M Hoffman / Amy C Rosenzweig /
PubMed 要旨	Nature's primary methane-oxidizing enzyme, the membrane-bound particulate methane monooxygenase (pMMO), catalyzes the oxidation of methane to methanol. pMMO activity requires copper, and decades of ...Nature's primary methane-oxidizing enzyme, the membrane-bound particulate methane monooxygenase (pMMO), catalyzes the oxidation of methane to methanol. pMMO activity requires copper, and decades of structural and spectroscopic studies have sought to identify the active site among three candidates: the Cu, Cu, and Cu sites. Challenges associated with the isolation of active pMMO have hindered progress toward locating its catalytic center. However, reconstituting pMMO into native lipid nanodiscs stabilizes its structure and recovers its activity. Here, these active samples were incubated with 2,2,2,-trifluoroethanol (TFE), a product analog that serves as a readily visualized active-site probe. Interactions of TFE with the Cu site were observed by both pulsed ENDOR spectroscopy and cryoEM, implicating Cu and the surrounding hydrophobic pocket as the likely site of methane oxidation. Use of these orthogonal techniques on parallel samples is a powerful approach that can circumvent difficulties in interpreting metalloenzyme cryoEM maps.
リンク	Nat Catal / PubMed:38187819 / PubMed Central
手法	EM (単粒子)
解像度	2.16 - 3.22 Å
構造データ	17287 8oyi EMDB-17287, PDB-8oyi: particulate methane monooxygenase with 2,2,2-trifluoroethanol bound 手法: EM (単粒子) / 解像度: 2.19 Å 40714 8sqw EMDB-40714, PDB-8sqw: particulate methane monooxygenase crosslinked with 2,2,2-trifluoroethanol bound 手法: EM (単粒子) / 解像度: 2.16 Å 40717 8sr1 EMDB-40717, PDB-8sr1: particulate methane monooxygenase crosslinked with 4,4,4-trifluorobutanol bound 手法: EM (単粒子) / 解像度: 2.18 Å 40719 8sr4 EMDB-40719, PDB-8sr4: particulate methane monooxygeanse treated with potassium cyanide and copper reloaded 手法: EM (単粒子) / 解像度: 3.12 Å 40720 8sr5 EMDB-40720, PDB-8sr5: particulate methane monooxygenase potassium cyanide treated 手法: EM (単粒子) / 解像度: 3.22 Å
化合物	ChemComp-CU: COPPER (II) ION ChemComp-D10: DECANE / デカン ChemComp-PLC: DIUNDECYL PHOSPHATIDYL CHOLINE / O-(1-O,2-O-ジドデカノイル-L-グリセロ-3-ホスホ)コリン / リン脂質YM ChemComp-P1O: 1,2-DIDECANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / ジデカノイルホスファチジルコリン / DDPC, リン脂質YM ChemComp-HXG: 1,2-dihexanoyl-sn-glycero-3-phosphocholine / O-(1-O,2-O-ジヘキサノイル-L-グリセロ-3-ホスホ)コリン ChemComp-ETF: TRIFLUOROETHANOL / 2,2,2-トリフルオロエタノ-ル ChemComp-HOH: WATER ChemComp-WIY: 4,4,4-trifluorobutan-1-ol / 4,4,4-トリフルオロ-1-ブタノ-ル
由来	methylococcus capsulatus str. bath (バクテリア) methylococcus capsulatus (バクテリア)
キーワード	OXIDOREDUCTASE / Metalloenzyme / Membrane Protein / Inhibitor / Nanodisc / Nanodiscs / Active Site