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TitleLight-induced Trp in /Met out Switching During BLUF Domain Activation in ATP-bound Photoactivatable Adenylate Cyclase OaPAC.
Journal, issue, pagesJ. Mol. Biol., Vol. 436, Page 168439-168439, Year 2024
Publish dateSep 4, 2023 (structure data deposition date)
AuthorsChretien, A. / Nagel, M.F. / Botha, S. / de Wijn, R. / Brings, L. / Dorner, K. / Han, H. / Koliyadu, J.C.P. / Letrun, R. / Round, A. ...Chretien, A. / Nagel, M.F. / Botha, S. / de Wijn, R. / Brings, L. / Dorner, K. / Han, H. / Koliyadu, J.C.P. / Letrun, R. / Round, A. / Sato, T. / Schmidt, C. / Secareanu, R.C. / von Stetten, D. / Vakili, M. / Wrona, A. / Bean, R. / Mancuso, A. / Schulz, J. / Pearson, A.R. / Kottke, T. / Lorenzen, K. / Schubert, R.
External linksJ. Mol. Biol. / PubMed:38185322
MethodsX-ray diffraction
Resolution1.5 - 2.1 Å
Structure data

PDB-8qfe:
Cryogenic crystal structure of the Photoactivated Adenylate Cyclase OaPAC
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-8qff:
Cryogenic crystal structure of the Photoactivated Adenylate Cyclase OaPAC with ATP bound
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-8qfg:
Cryogenic crystal structure of the Photoactivated Adenylate Cyclase OaPAC after 5 seconds of blue light illumination
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-8qfh:
Room temperature crystal structure of the Photoactivated Adenylate Cyclase OaPAC with ATP bound
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-8qfi:
Room temperature crystal structure of the Photoactivated Adenylate Cyclase OaPAC after blue light excitation at 1.8 us delay
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-8qfj:
Room temperature crystal structure of the Photoactivated Adenylate Cyclase OaPAC after blue light excitation at 2.3 us delay
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • oscillatoria acuminata pcc 6304 (bacteria)
KeywordsLYASE / Photoreceptor / BLUF / Adenylate Cyclase / PAC

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