EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures and roles of BcsD and partner scaffold proteins in proteobacterial cellulose secretion.
ジャーナル・号・ページ	Curr Biol, Vol. 34, Issue 1, Page 106-116.e6, Year 2024
掲載日	2024年1月8日
著者	Thibault G Sana / Areti Notopoulou / Lucie Puygrenier / Marion Decossas / Sandra Moreau / Aurélien Carlier / Petya V Krasteva /
PubMed 要旨	Cellulose is the world's most abundant biopolymer, and similar to its role as a cell wall component in plants, it is a prevalent constituent of the extracellular matrix in bacterial biofilms. ...Cellulose is the world's most abundant biopolymer, and similar to its role as a cell wall component in plants, it is a prevalent constituent of the extracellular matrix in bacterial biofilms. Although bacterial cellulose (BC) was first described in the 19 century, it was only recently revealed that it is produced by several distinct types of Bcs secretion systems that feature multiple accessory subunits in addition to a catalytic BcsAB synthase tandem. We recently showed that crystalline cellulose secretion in the Gluconacetobacter genus (α-Proteobacteria) is driven by a supramolecular BcsH-BcsD scaffold-the "cortical belt"-which stabilizes the synthase nanoarrays through an unexpected inside-out mechanism for secretion system assembly. Interestingly, while bcsH is specific for Gluconacetobacter, bcsD homologs are widespread in Proteobacteria. Here, we examine BcsD homologs and their gene neighborhoods from several plant-colonizing β- and γ-Proteobacteria proposed to secrete a variety of non-crystalline and/or chemically modified cellulosic polymers. We provide structural and mechanistic evidence that through different quaternary structure assemblies BcsD acts with proline-rich BcsH, BcsP, or BcsO partners across the proteobacterial clade to form synthase-interacting intracellular scaffolds that, in turn, determine the biofilm strength and architecture in species with strikingly different physiology and secreted biopolymers.
リンク	Curr Biol / PubMed:38141614
手法	EM (単粒子)
解像度	2.33 - 4.15 Å
構造データ	17735 8pkd EMDB-17735, PDB-8pkd: Cryo-EM structure of Orrella dioscoreae BcsD 手法: EM (単粒子) / 解像度: 2.33 Å 17788 8poc EMDB-17788, PDB-8poc: Cryo-EM structure of Dickeya dadantii BcsD 手法: EM (単粒子) / 解像度: 4.0 Å 17791 8pog EMDB-17791, PDB-8pog: Cryo-EM structure of Enterobacter sp. 638 BcsD 手法: EM (単粒子) / 解像度: 4.15 Å
化合物	ChemComp-HOH: WATER / 水
由来	orrella dioscoreae (バクテリア) dickeya dadantii 3937 (バクテリア) enterobacter sp. 638 (バクテリア)
キーワード	STRUCTURAL PROTEIN (タンパク質) / Bacterial cytoskeleton (原核生物の細胞骨格) / bacterial cellulose / bacterial secretion / bacterial biofilms / CYTOSOLIC PROTEIN (細胞質基質) / Cellulose secretion / cytoskeleton (細胞骨格)