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Structure paper

TitleDiscovery of a non-canonical prototype long-chain monoacylglycerol lipase through a structure-based endogenous reaction intermediate complex.
Journal, issue, pagesNat Commun, Vol. 14, Page 7649-7649, Year 2023
Publish dateOct 30, 2021 (structure data deposition date)
AuthorsPinotsis, N. / Kruger, A. / Tomas, N. / Chatziefthymiou, S.D. / Litz, C. / Mortensen, S.A. / Daffe, M. / Marrakchi, H. / Antranikian, G. / Wilmanns, M.
External linksNat Commun / PubMed:38012138
MethodsX-ray diffraction
Resolution1.91 - 2.42 Å
Structure data

PDB-7q4h:
A thermostable lipase from Thermoanaerobacter thermohydrosulfuricus in complex with PMSF
Method: X-RAY DIFFRACTION / Resolution: 2.00003223771 Å

PDB-7q4j:
A thermostable lipase from Thermoanaerobacter thermohydrosulfuricus in complex a monoacylglycerol intermediate
Method: X-RAY DIFFRACTION / Resolution: 1.91 Å

PDB-8b9s:
NATIVE FORM, THERMOSTABLE LIPASE FROM THERMOANAEROBACTER THERMOHYDROSULFURICUS
Method: X-RAY DIFFRACTION / Resolution: 2.42 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-SO4:
SULFATE ION

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-HOH:
WATER

ChemComp-STE:
STEARIC ACID

ChemComp-OCT:
N-OCTANE

ChemComp-DD9:
nonane

ChemComp-D10:
DECANE

ChemComp-PO4:
PHOSPHATE ION

ChemComp-K:
Unknown entry

Source
  • thermoanaerobacter thermohydrosulfuricus (bacteria)
KeywordsHYDROLASE / ALPHA/BETA HYDROLASE / THERMOSTABLE / PMSF / monoacylglycerol / lipase

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