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TitleStructure of the transcription open complex of distinct σ factors.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 6455, Year 2023
Publish dateOct 13, 2023
AuthorsJie Li / Haonan Zhang / Dongyu Li / Ya-Jun Liu / Edward A Bayer / Qiu Cui / Yingang Feng / Ping Zhu /
PubMed AbstractBacterial σ factors of the σ-family are widespread in Bacilli and Clostridia and are involved in the heat shock response, iron metabolism, virulence, and carbohydrate sensing. A multiplicity of σ ...Bacterial σ factors of the σ-family are widespread in Bacilli and Clostridia and are involved in the heat shock response, iron metabolism, virulence, and carbohydrate sensing. A multiplicity of σ paralogues in some cellulolytic bacteria have been shown to be responsible for the regulation of the cellulosome, a multienzyme complex that mediates efficient cellulose degradation. Here, we report two structures at 3.0 Å and 3.3 Å of two transcription open complexes formed by two σ factors, SigI1 and SigI6, respectively, from the thermophilic, cellulolytic bacterium, Clostridium thermocellum. These structures reveal a unique, hitherto-unknown recognition mode of bacterial transcriptional promoters, both with respect to domain organization and binding to promoter DNA. The key characteristics that determine the specificities of the σ paralogues were further revealed by comparison of the two structures. Consequently, the σ factors represent a distinct set of the σ-family σ factors, thus highlighting the diversity of bacterial transcription.
External linksNat Commun / PubMed:37833284 / PubMed Central
MethodsEM (single particle)
Resolution3.03 - 3.36 Å
Structure data

35130

8i23

EMDB-35130, PDB-8i23:
Clostridium thermocellum RNA polymerase transcription open complex with SigI1 and its promoter
Method: EM (single particle) / Resolution: 3.03 Å

35131

8i24

EMDB-35131, PDB-8i24:
Clostridium thermocellum RNA polymerase transcription open complex with SigI6 and its promoter
Method: EM (single particle) / Resolution: 3.36 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • acetivibrio thermocellus dsm 1313 (bacteria)
  • acetivibrio thermocellus dsm1313 (bacteria)
  • acetivibrio thermocellus (bacteria)
KeywordsTRANSCRIPTION/DNA / TRANSCRIPTION OPEN COMPLEX / SIGI / TRANSCRIPTION / TRANSCRIPTION-DNA complex

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