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Structure paper

TitleStructural insights into the mechanism of GTP initiation of microtubule assembly.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 5980, Year 2023
Publish dateSep 25, 2023
AuthorsJu Zhou / Anhui Wang / Yinlong Song / Nan Liu / Jia Wang / Yan Li / Xin Liang / Guohui Li / Huiying Chu / Hong-Wei Wang /
PubMed AbstractIn eukaryotes, the dynamic assembly of microtubules (MT) plays an important role in numerous cellular processes. The underlying mechanism of GTP triggering MT assembly is still unknown. Here, we ...In eukaryotes, the dynamic assembly of microtubules (MT) plays an important role in numerous cellular processes. The underlying mechanism of GTP triggering MT assembly is still unknown. Here, we present cryo-EM structures of tubulin heterodimer at their GTP- and GDP-bound states, intermediate assembly states of GTP-tubulin, and final assembly stages of MT. Both GTP- and GDP-tubulin heterodimers adopt similar curved conformations with subtle flexibility differences. In head-to-tail oligomers of tubulin heterodimers, the inter-dimer interface of GDP-tubulin exhibits greater flexibility, particularly in tangential bending. Cryo-EM of the intermediate assembly states reveals two types of tubulin lateral contacts, "Tube-bond" and "MT-bond". Further, molecular dynamics (MD) simulations show that GTP triggers lateral contact formation in MT assembly in multiple sequential steps, gradually straightening the curved tubulin heterodimers. Therefore, we propose a flexible model of GTP-initiated MT assembly, including the formation of longitudinal and lateral contacts, to explain the nucleation and assembly of MT.
External linksNat Commun / PubMed:37749104 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution3.5 - 6.8 Å
Structure data

34077

7ysn

EMDB-34077, PDB-7ysn:
Tubulin heterodimer structure of GMPCPP state in solution
Method: EM (single particle) / Resolution: 3.5 Å

34078

7yso

EMDB-34078, PDB-7yso:
Tubulin heterodimer structure of GDP-1 state in solution
Method: EM (single particle) / Resolution: 3.6 Å

34079

7ysp

EMDB-34079, PDB-7ysp:
Tubulin heterodimer structure of GDP-2 state in solution
Method: EM (single particle) / Resolution: 3.9 Å

34080

7ysq

EMDB-34080, PDB-7ysq:
GTPgammaS Tube decorated with kinesin
Method: EM (helical sym.) / Resolution: 6.8 Å

34081

7ysr

EMDB-34081, PDB-7ysr:
GTPgammaS MT decorated with kinesin
Method: EM (helical sym.) / Resolution: 4.3 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-GSP:
5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

Source
  • sus scrofa (pig)
  • drosophila melanogaster (fruit fly)
KeywordsSTRUCTURAL PROTEIN / cytoskeleton / PROTEIN FIBRIL / Complex

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