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TitleStructure of a fungal 1,3-β-glucan synthase.
Journal, issue, pagesSci Adv, Vol. 9, Issue 37, Page eadh7820, Year 2023
Publish dateSep 15, 2023
AuthorsChao-Ran Zhao / Zi-Long You / Dan-Dan Chen / Jing Hang / Zhao-Bin Wang / Meng Ji / Le-Xuan Wang / Peng Zhao / Jie Qiao / Cai-Hong Yun / Lin Bai /
PubMed Abstract1,3-β-Glucan serves as the primary component of the fungal cell wall and is produced by 1,3-β-glucan synthase located in the plasma membrane. This synthase is a molecular target for antifungal ...1,3-β-Glucan serves as the primary component of the fungal cell wall and is produced by 1,3-β-glucan synthase located in the plasma membrane. This synthase is a molecular target for antifungal drugs such as echinocandins and the triterpenoid ibrexafungerp. In this study, we present the cryo-electron microscopy structure of 1,3-β-glucan synthase (Fks1) at 2.47-Å resolution. The structure reveals a central catalytic region adopting a cellulose synthase fold with a cytosolic conserved GT-A-type glycosyltransferase domain and a closed transmembrane channel responsible for glucan transportation. Two extracellular disulfide bonds are found to be crucial for Fks1 enzymatic activity. Through structural comparative analysis with cellulose synthases and structure-guided mutagenesis studies, we gain previously unknown insights into the molecular mechanisms of fungal 1,3-β-glucan synthase.
External linksSci Adv / PubMed:37703377 / PubMed Central
MethodsEM (single particle)
Resolution2.47 Å
Structure data

36748

8jzn

EMDB-36748, PDB-8jzn:
Structure of a fungal 1,3-beta-glucan synthase
Method: EM (single particle) / Resolution: 2.47 Å

Chemicals

ChemComp-POV:
(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / phospholipid*YM

ChemComp-AV0:
Lauryl Maltose Neopentyl Glycol

ChemComp-ERG:
ERGOSTEROL

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsTRANSFERASE / fungi / drug target

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