+検索条件
-Structure paper
タイトル | Structural and antigenic characterization of the avian adeno-associated virus capsid. |
---|---|
ジャーナル・号・ページ | J Virol, Vol. 97, Issue 10, Page e0078023, Year 2023 |
掲載日 | 2023年10月31日 |
著者 | Jane Hsi / Mario Mietzsch / Paul Chipman / Sandra Afione / Allison Zeher / Rick Huang / John Chiorini / Robert McKenna / |
PubMed 要旨 | AAVs are extensively studied as promising therapeutic gene delivery vectors. In order to circumvent pre-existing antibodies targeting primate-based AAV capsids, the AAAV capsid was evaluated as an ...AAVs are extensively studied as promising therapeutic gene delivery vectors. In order to circumvent pre-existing antibodies targeting primate-based AAV capsids, the AAAV capsid was evaluated as an alternative to primate-based therapeutic vectors. Despite the high sequence diversity, the AAAV capsid was found to bind to a common glycan receptor, terminal galactose, which is also utilized by other AAVs already being utilized in gene therapy trials. However, contrary to the initial hypothesis, AAAV was recognized by approximately 30% of human sera tested. Structural and sequence comparisons point to conserved epitopes in the fivefold region of the capsid as the reason determinant for the observed cross-reactivity. |
リンク | J Virol / PubMed:37702486 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.54 - 3.06 Å |
構造データ | EMDB-41208, PDB-8tex: EMDB-41209, PDB-8tey: |
化合物 | ChemComp-D5M: |
由来 |
|
キーワード | VIRUS / Adeno-associated virus / capsid / quail bronchitis / avianAAV / AAAV |