EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of liganded glycosylphosphatidylinositol transamidase illuminate GPI-AP biogenesis.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 5520, Year 2023
掲載日	2023年9月8日
著者	Yidan Xu / Tingting Li / Zixuan Zhou / Jingjing Hong / Yulin Chao / Zhini Zhu / Ying Zhang / Qianhui Qu / Dianfan Li /
PubMed 要旨	Many eukaryotic receptors and enzymes rely on glycosylphosphatidylinositol (GPI) anchors for membrane localization and function. The transmembrane complex GPI-T recognizes diverse proproteins at a ...Many eukaryotic receptors and enzymes rely on glycosylphosphatidylinositol (GPI) anchors for membrane localization and function. The transmembrane complex GPI-T recognizes diverse proproteins at a signal peptide region that lacks consensus sequence and replaces it with GPI via a transamidation reaction. How GPI-T maintains broad specificity while preventing unintentional cleavage is unclear. Here, substrates- and products-bound human GPI-T structures identify subsite features that enable broad proprotein specificity, inform catalytic mechanism, and reveal a multilevel safeguard mechanism against its promiscuity. In the absence of proproteins, the catalytic site is invaded by a locally stabilized loop. Activation requires energetically unfavorable rearrangements that transform the autoinhibitory loop into crucial catalytic cleft elements. Enzyme-proprotein binding in the transmembrane and luminal domains respectively powers the conformational rearrangement and induces a competent cleft. GPI-T thus integrates various weak specificity regions to form strong selectivity and prevent accidental activation. These findings provide important mechanistic insights into GPI-anchored protein biogenesis.
リンク	Nat Commun / PubMed:37684232 / PubMed Central
手法	EM (単粒子)
解像度	2.85 - 3.22 Å
構造データ	35575 8imx EMDB-35575, PDB-8imx: Cryo-EM structure of GPI-T with a chimeric GPI-anchored protein 手法: EM (単粒子) / 解像度: 2.85 Å 35576 8imy EMDB-35576, PDB-8imy: Cryo-EM structure of GPI-T (inactive mutant) with GPI and proULBP2, a proprotein substrate 手法: EM (単粒子) / 解像度: 3.22 Å
化合物	ChemComp-Y01: CHOLESTEROL HEMISUCCINATE / コレステリルヘミスクシナ-ト ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-6OU: [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate / POPE / リン脂質YM ChemComp-AJP: Digitonin / ジギトニン / 可溶化剤YM ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-LBN: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine / リン脂質YM ChemComp-80T: [(2R)-1-hexadecanoyloxy-3-[[3-[[(2R)-3-hexadecanoyloxy-2-[(Z)-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propan-2-yl] (Z)-octadec-9-enoate ChemComp-80Y: 2-azanylethyl [(2R,3S,4S,5S,6S)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphate ChemComp-PA1: 2-amino-2-deoxy-alpha-D-glucopyranose / α-D-グルコサミン ChemComp-05E: 2-azanylethyl [(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate ChemComp-81Q*: [(2R)-1-[[(1S,2R,3R,4S,5S,6R)-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-3-octadecoxy-propan-2-yl] (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate
由来	homo sapiens (ヒト) clavularia sp. (無脊椎動物) synthetic construct (人工物)
キーワード	MEMBRANE PROTEIN / cryo-EM / glycosylphosphatidylinositol / GPI / GPI anchored protein / GPI-AP / membrane protein complex / proprotein