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- PDB-8imx: Cryo-EM structure of GPI-T with a chimeric GPI-anchored protein -

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Entry
Database: PDB / ID: 8imx
TitleCryo-EM structure of GPI-T with a chimeric GPI-anchored protein
Components
  • (GPI transamidase component PIG- ...) x 2
  • GPI-anchor transamidase,GFP-like fluorescent chromoprotein cFP484
  • Glycosylphosphatidylinositol anchor attachment 1 protein,GFP-like fluorescent chromoprotein cFP484
  • Met-Ser-Ser peptide
  • Phosphatidylinositol glycan anchor biosynthesis class U protein,GFP-like fluorescent chromoprotein cFP484
  • UL16-binding protein 2,GFP-like fluorescent chromoprotein cFP484
KeywordsMEMBRANE PROTEIN / cryo-EM / glycosylphosphatidylinositol / GPI / GPI anchored protein / GPI-AP / membrane protein complex
Function / homology
Function and homology information


GPI-anchor transamidase activity / attachment of GPI anchor to protein / GPI-anchor transamidase complex / GPI anchored protein biosynthesis / GPI anchor biosynthetic process / GPI anchor binding / Attachment of GPI anchor to uPAR / protein retention in ER lumen / natural killer cell lectin-like receptor binding / Transferases; Transferring nitrogenous groups; Transaminases ...GPI-anchor transamidase activity / attachment of GPI anchor to protein / GPI-anchor transamidase complex / GPI anchored protein biosynthesis / GPI anchor biosynthetic process / GPI anchor binding / Attachment of GPI anchor to uPAR / protein retention in ER lumen / natural killer cell lectin-like receptor binding / Transferases; Transferring nitrogenous groups; Transaminases / Post-translational modification: synthesis of GPI-anchored proteins / natural killer cell activation / regulation of receptor signaling pathway via JAK-STAT / natural killer cell mediated cytotoxicity / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / bioluminescence / generation of precursor metabolites and energy / positive regulation of T cell mediated cytotoxicity / neuron differentiation / cytoplasmic vesicle / neuron apoptotic process / immune response / receptor ligand activity / external side of plasma membrane / intracellular membrane-bounded organelle / centrosome / endoplasmic reticulum membrane / cell surface / endoplasmic reticulum / mitochondrion / proteolysis / extracellular space / extracellular region / membrane / plasma membrane / cytosol
Similarity search - Function
GPI transamidase component PIG-T / GPI transamidase component Gaa1 / GPI transamidase subunit PIG-U / Phosphatidylinositol-glycan biosynthesis class S protein / GPI-anchor transamidase / Gpi16 subunit, GPI transamidase component / Gaa1-like, GPI transamidase component / GPI transamidase subunit PIG-U / Phosphatidylinositol-glycan biosynthesis class S protein / Peptidase C13, legumain ...GPI transamidase component PIG-T / GPI transamidase component Gaa1 / GPI transamidase subunit PIG-U / Phosphatidylinositol-glycan biosynthesis class S protein / GPI-anchor transamidase / Gpi16 subunit, GPI transamidase component / Gaa1-like, GPI transamidase component / GPI transamidase subunit PIG-U / Phosphatidylinositol-glycan biosynthesis class S protein / Peptidase C13, legumain / Peptidase C13 family / Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / Class I Histocompatibility antigen, domains alpha 1 and 2 / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein
Similarity search - Domain/homology
Chem-05E / Chem-6OU / Chem-80T / Chem-80Y / Chem-81Q / 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine / 2-amino-2-deoxy-alpha-D-glucopyranose / CHOLESTEROL HEMISUCCINATE / GPI-anchor transamidase component GPAA1 / GPI-anchor transamidase ...Chem-05E / Chem-6OU / Chem-80T / Chem-80Y / Chem-81Q / 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine / 2-amino-2-deoxy-alpha-D-glucopyranose / CHOLESTEROL HEMISUCCINATE / GPI-anchor transamidase component GPAA1 / GPI-anchor transamidase / GPI-anchor transamidase component PIGT / GPI-anchor transamidase component PIGS / UL16-binding protein 2 / GPI-anchor transamidase component PIGU / GFP-like fluorescent chromoprotein cFP484
Similarity search - Component
Biological speciesHomo sapiens (human)
Clavularia sp. (invertebrata)
synthetic construct (others)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.85 Å
AuthorsXu, Y. / Li, T. / Qu, Q. / Li, D.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)82151215 China
CitationJournal: Nat Commun / Year: 2023
Title: Structures of liganded glycosylphosphatidylinositol transamidase illuminate GPI-AP biogenesis.
Authors: Yidan Xu / Tingting Li / Zixuan Zhou / Jingjing Hong / Yulin Chao / Zhini Zhu / Ying Zhang / Qianhui Qu / Dianfan Li /
Abstract: Many eukaryotic receptors and enzymes rely on glycosylphosphatidylinositol (GPI) anchors for membrane localization and function. The transmembrane complex GPI-T recognizes diverse proproteins at a ...Many eukaryotic receptors and enzymes rely on glycosylphosphatidylinositol (GPI) anchors for membrane localization and function. The transmembrane complex GPI-T recognizes diverse proproteins at a signal peptide region that lacks consensus sequence and replaces it with GPI via a transamidation reaction. How GPI-T maintains broad specificity while preventing unintentional cleavage is unclear. Here, substrates- and products-bound human GPI-T structures identify subsite features that enable broad proprotein specificity, inform catalytic mechanism, and reveal a multilevel safeguard mechanism against its promiscuity. In the absence of proproteins, the catalytic site is invaded by a locally stabilized loop. Activation requires energetically unfavorable rearrangements that transform the autoinhibitory loop into crucial catalytic cleft elements. Enzyme-proprotein binding in the transmembrane and luminal domains respectively powers the conformational rearrangement and induces a competent cleft. GPI-T thus integrates various weak specificity regions to form strong selectivity and prevent accidental activation. These findings provide important mechanistic insights into GPI-anchored protein biogenesis.
History
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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
D: UL16-binding protein 2,GFP-like fluorescent chromoprotein cFP484
G: Glycosylphosphatidylinositol anchor attachment 1 protein,GFP-like fluorescent chromoprotein cFP484
K: GPI-anchor transamidase,GFP-like fluorescent chromoprotein cFP484
S: GPI transamidase component PIG-S,GFP-like fluorescent chromoprotein cFP484
T: GPI transamidase component PIG-T,GFP-like fluorescent chromoprotein cFP484
U: Phosphatidylinositol glycan anchor biosynthesis class U protein,GFP-like fluorescent chromoprotein cFP484
P: Met-Ser-Ser peptide
hetero molecules


Theoretical massNumber of molelcules
Total (without water)483,82637
Polymers468,1007
Non-polymers15,72630
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Protein , 4 types, 4 molecules DGKU

#1: Protein UL16-binding protein 2,GFP-like fluorescent chromoprotein cFP484 / ALCAN-alpha / NKG2D ligand 2 / N2DL-2 / NKG2DL2 / Retinoic acid early transcript 1H


Mass: 35573.316 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: A chimera proprotein containing the N- and C-terminal signal peptide of ULBP2, and a thermostable green fluorescence protein replacing the main part of ULBP2.
Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Clavularia sp. (invertebrata)
Gene: ULBP2, N2DL2, RAET1H, UNQ463/PRO791 / Production host: Homo sapiens (human) / References: UniProt: Q9BZM5, UniProt: Q9U6Y3
#2: Protein Glycosylphosphatidylinositol anchor attachment 1 protein,GFP-like fluorescent chromoprotein cFP484 / GPI anchor attachment protein 1 / GAA1 protein homolog / hGAA1


Mass: 96990.352 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Clavularia sp. (invertebrata)
Gene: GPAA1, GAA1 / Production host: Homo sapiens (human) / References: UniProt: O43292, UniProt: Q9U6Y3
#3: Protein GPI-anchor transamidase,GFP-like fluorescent chromoprotein cFP484 / GPI transamidase / GPI8 homolog / hGPI8 / Phosphatidylinositol-glycan biosynthesis class K protein / PIG-K


Mass: 73443.062 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Clavularia sp. (invertebrata)
Gene: PIGK, GPI8 / Production host: Homo sapiens (human) / References: UniProt: Q92643, UniProt: Q9U6Y3, Hydrolases
#6: Protein Phosphatidylinositol glycan anchor biosynthesis class U protein,GFP-like fluorescent chromoprotein cFP484 / Cell division cycle protein 91-like 1 / Protein CDC91-like 1 / GPI transamidase component PIG-U


Mass: 77141.695 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Clavularia sp. (invertebrata)
Gene: PIGU, CDC91L1, PSEC0205, UNQ3055/PRO9875 / Production host: Homo sapiens (human) / References: UniProt: Q9H490, UniProt: Q9U6Y3

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GPI transamidase component PIG- ... , 2 types, 2 molecules ST

#4: Protein GPI transamidase component PIG-S,GFP-like fluorescent chromoprotein cFP484 / Phosphatidylinositol-glycan biosynthesis class S protein


Mass: 90421.062 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Clavularia sp. (invertebrata)
Gene: PIGS, UNQ1873/PRO4316 / Production host: Homo sapiens (human) / References: UniProt: Q96S52, UniProt: Q9U6Y3
#5: Protein GPI transamidase component PIG-T,GFP-like fluorescent chromoprotein cFP484 / Phosphatidylinositol-glycan biosynthesis class T protein


Mass: 94207.250 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human), (gene. exp.) Clavularia sp. (invertebrata)
Gene: PIGT, CGI-06, PSEC0163, UNQ716/PRO1379 / Production host: Homo sapiens (human) / References: UniProt: Q969N2, UniProt: Q9U6Y3

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Protein/peptide , 1 types, 1 molecules P

#7: Protein/peptide Met-Ser-Ser peptide


Mass: 323.367 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Sugars , 3 types, 4 molecules

#8: Polysaccharide 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}LINUCSPDB-CARE
#14: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
#18: Sugar ChemComp-PA1 / 2-amino-2-deoxy-alpha-D-glucopyranose / alpah-D-glucosamine / 2-amino-2-deoxy-alpha-D-glucose / 2-amino-2-deoxy-D-glucose / 2-amino-2-deoxy-glucose


Type: D-saccharide, alpha linking / Mass: 179.171 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Formula: C6H13NO5 / Feature type: SUBJECT OF INVESTIGATION
IdentifierTypeProgram
DGlcpNaCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
a-D-glucopyranosamineCOMMON NAMEGMML 1.0
a-D-GlcpNIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 10 types, 26 molecules

#9: Chemical
ChemComp-Y01 / CHOLESTEROL HEMISUCCINATE


Mass: 486.726 Da / Num. of mol.: 13 / Source method: obtained synthetically / Formula: C31H50O4
#10: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg
#11: Chemical
ChemComp-6OU / [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate


Mass: 717.996 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C39H76NO8P / Comment: phospholipid*YM
#12: Chemical ChemComp-AJP / Digitonin


Mass: 1229.312 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C56H92O29 / Comment: detergent*YM
#13: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Ca
#15: Chemical ChemComp-LBN / 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine / (2R)-2-[(9Z)-9-Octadecenoyloxy]-3-(palmitoyloxy)propyl 2-(trimethylammonio)ethyl phosphate


Mass: 760.076 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C42H82NO8P / Comment: phospholipid*YM
#16: Chemical ChemComp-80T / [(2R)-1-hexadecanoyloxy-3-[[3-[[(2R)-3-hexadecanoyloxy-2-[(Z)-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propan-2-yl] (Z)-octadec-9-enoate


Mass: 1405.920 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C77H146O17P2
#17: Chemical ChemComp-80Y / 2-azanylethyl [(2R,3S,4S,5S,6S)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphate


Mass: 303.204 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H18NO9P / Feature type: SUBJECT OF INVESTIGATION
#19: Chemical ChemComp-05E / 2-azanylethyl [(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate


Mass: 303.204 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C8H18NO9P / Feature type: SUBJECT OF INVESTIGATION
#20: Chemical ChemComp-81Q / [(2R)-1-[[(1S,2R,3R,4S,5S,6R)-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-3-octadecoxy-propan-2-yl] (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate


Mass: 1111.553 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C63H115O13P / Feature type: SUBJECT OF INVESTIGATION

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Complex of GPI-T with a chimeric GPI-anchored protein / Type: COMPLEX / Entity ID: #1-#6 / Source: MULTIPLE SOURCES
Molecular weightValue: 469 kDa/nm / Experimental value: NO
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Homo sapiens (human)
Buffer solutionpH: 8
Details: 0.1 % Digitonin, 150 mM NaCl, 50 mM Tris-HCl, pH 8.0
Buffer component
IDConc.NameFormulaBuffer-ID
10.15 MSodium chlorideNaCl1
20.1 % (w/v)DigitoninDigitonin1
30.05 MTris(hydroxymethyl)aminomethaneTris1
SpecimenConc.: 8 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: GOLD / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm / Cs: 2.7 mm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k)

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Processing

SoftwareName: PHENIX / Version: 1.20.1_4487: / Classification: refinement
EM software
IDNameVersionCategory
1cryoSPARCv4.2.0particle selection
2EPUv3.0.0.4164RELimage acquisition
4CTFFINDv4.0CTF correction
8PHENIXmodel refinement
10cryoSPARCv4.2.0initial Euler assignment
11cryoSPARCv4.2.0final Euler assignment
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNum. of particles selected: 3170202
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionResolution: 2.85 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 34261 / Algorithm: FOURIER SPACE / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.00420414
ELECTRON MICROSCOPYf_angle_d0.58327900
ELECTRON MICROSCOPYf_dihedral_angle_d12.8933473
ELECTRON MICROSCOPYf_chiral_restr0.0413295
ELECTRON MICROSCOPYf_plane_restr0.0073370

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