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Structure paper

TitleIn search for structural targets for engineering d-amino acid transaminase: modulation of pH optimum and substrate specificity.
Journal, issue, pagesBiochem. J., Vol. 480, Page 1267-1284, Year 2023
Publish dateSep 2, 2022 (structure data deposition date)
AuthorsShilova, S.A. / Matyuta, I.O. / Khrenova, M.G. / Nikolaeva, A.Y. / Klyachko, N.L. / Minyaev, M.E. / Khomutov, A.R. / Boyko, K.M. / Popov, V.O. / Bezsudnova, E.Y.
External linksBiochem. J. / PubMed:37548495
MethodsX-ray diffraction
Resolution1.75 - 2.1 Å
Structure data

PDB-8ayj:
Crystal structure of D-amino acid aminotransferase from Aminobacterium colombiens complexed with 3-aminooxypropionic acid
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-8onj:
Crystal structure of D-amino acid aminotransferase from Aminobacterium colombiense point mutant R88L
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-8onl:
Crystal structure of D-amino acid aminotransferase from Aminobacterium colombiense point mutant E113A
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-8onn:
Crystal structure of D-amino acid aminotransferase from Aminobacterium colombiense point mutant E113A complexed with 3-aminooxypropionic acid
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-OCF:
3-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]oxypropanoic acid

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE

ChemComp-HOH:
WATER

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • aminobacterium colombiense (bacteria)
KeywordsTRANSFERASE / Transaminase / DAAT / aminotransferase / complex / oxyme / 3-aminooxypropionic acid / Aminobacterium colombiens / point mutant

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