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TitleStructural insights into anion selectivity and activation mechanism of LRRC8 volume-regulated anion channels.
Journal, issue, pagesCell Rep, Vol. 42, Issue 8, Page 112926, Year 2023
Publish dateAug 29, 2023
AuthorsHeng Liu / Maya M Polovitskaya / Linlin Yang / Meiling Li / Hongyue Li / Zhen Han / Jianguo Wu / Qiansen Zhang / Thomas J Jentsch / Jun Liao /
PubMed AbstractVolume-regulated anion channels (VRACs) are hexamers of LRRC8 proteins that are crucial for cell volume regulation. N termini (NTs) of the obligatory LRRC8A subunit modulate VRACs activation and ion ...Volume-regulated anion channels (VRACs) are hexamers of LRRC8 proteins that are crucial for cell volume regulation. N termini (NTs) of the obligatory LRRC8A subunit modulate VRACs activation and ion selectivity, but the underlying mechanisms remain poorly understood. Here, we report a 2.8-Å cryo-electron microscopy structure of human LRRC8A that displays well-resolved NTs. Amino-terminal halves of NTs fold back into the pore and constrict the permeation path, thereby determining ion selectivity together with an extracellular selectivity filter with which it works in series. They also interact with pore-surrounding helices and support their compact arrangement. The C-terminal halves of NTs interact with intracellular loops that are crucial for channel activation. Molecular dynamics simulations indicate that low ionic strength increases NT mobility and expands the radial distance between pore-surrounding helices. Our work suggests an unusual pore architecture with two selectivity filters in series and a mechanism for VRAC activation by cell swelling.
External linksCell Rep / PubMed:37543949 / PubMed Central
MethodsEM (single particle)
Resolution2.78 Å
Structure data

33527

7xzh

EMDB-33527, PDB-7xzh:
Cryo-EM structure of human LRRC8A
Method: EM (single particle) / Resolution: 2.78 Å

Chemicals

ChemComp-P5S:
O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine

ChemComp-POV:
(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / phospholipid*YM

ChemComp-CLR:
CHOLESTEROL

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / Volume Regulated Anion Channel / LRRC8A / Cryo-EM / VRAC / N terminal extention

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