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-Structure paper
Title | Structures and immune recognition of Env trimers from two Asia prevalent HIV-1 CRFs. |
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Journal, issue, pages | Nat Commun, Vol. 14, Issue 1, Page 4676, Year 2023 |
Publish date | Aug 4, 2023 |
Authors | Jun Niu / Qi Wang / Wenwen Zhao / Bing Meng / Youwei Xu / Xianfang Zhang / Yi Feng / Qilian Qi / Yanling Hao / Xuan Zhang / Ying Liu / Jiangchao Xiang / Yiming Shao / Bei Yang / |
PubMed Abstract | Structure-guided immunofocusing HIV-1 vaccine design entails a comprehensive understanding of Envs from diverse HIV-1 subtypes, including circulating recombinant forms (CRFs). Here, we present the ...Structure-guided immunofocusing HIV-1 vaccine design entails a comprehensive understanding of Envs from diverse HIV-1 subtypes, including circulating recombinant forms (CRFs). Here, we present the cryo-EM structures of Envs from two Asia prevalent CRFs (CRF01_AE and CRF07_BC) at 3.0 and 3.5 Å. We compare the structures and glycosylation patterns of Envs from different subtypes and perform cross-clade statistical analyses to reveal the unique features of CRF01_AE V1 region, which are associated with the resistance to certain bNAbs. We also solve a 4.1 Å cryo-EM structure of CRF01_AE Env in complex with F6, the first bNAb from CRF01_AE-infected individuals. F6 recognizes a gp120-gp41 spanning epitope to allosterically destabilize the Env trimer apex and weaken inter-protomer packing, which in turn hinders the receptor binding and induces Env trimer disassembly, demonstrating a dual mechanism of neutralization. These findings broaden our understanding of CRF Envs and shed lights on immunofocusing HIV-1 vaccine design. |
External links | Nat Commun / PubMed:37542068 / PubMed Central |
Methods | EM (single particle) / X-ray diffraction |
Resolution | 3.0 - 4.1 Å |
Structure data | EMDB-34190, PDB-8gp5: EMDB-34192, PDB-8gpg: EMDB-34193, PDB-8gpi: EMDB-34194, PDB-8gpj: PDB-8gpk: |
Chemicals | ChemComp-NAG: |
Source |
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Keywords | VIRAL PROTEIN / Antibody / HIV-1 / Env protein / Complex / IMMUNE SYSTEM / Fab / Env binding |