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-Structure paper
Title | Structure of mitoribosome reveals mechanism of mRNA binding, tRNA interactions with L1 stalk, roles of cofactors and rRNA modifications. |
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Journal, issue, pages | bioRxiv, Year 2023 |
Publish date | Jul 15, 2023 |
Authors | Vivek Singh / Yuzuru Itoh / Samuel Del'Olio / Asem Hassan / Andreas Naschberger / Rasmus Kock Flygaard / Yuko Nobe / Keiichi Izumikawa / Shintaro Aibara / Juni Andréll / Paul C Whitford / Antoni Barrientos / Masato Taoka / Alexey Amunts |
PubMed Abstract | The mitoribosome translates mitochondrial mRNAs and regulates energy conversion that is a signature of aerobic life forms. We present a 2.2 Å resolution structure of human mitoribosome together with ...The mitoribosome translates mitochondrial mRNAs and regulates energy conversion that is a signature of aerobic life forms. We present a 2.2 Å resolution structure of human mitoribosome together with validated mitoribosomal RNA (rRNA) modifications, including aminoacylated CP-tRNA . The structure shows how mitoribosomal proteins stabilise binding of mRNA and tRNA helping to align it in the decoding center, whereas the GDP-bound mS29 stabilizes intersubunit communication. Comparison between different states, with respect to tRNA position, allowed to characterize a non-canonical L1 stalk, and molecular dynamics simulations revealed how it facilitates tRNA transition in a way that does not require interactions with rRNA. We also report functionally important polyamines that are depleted when cells are subjected to an antibiotic treatment. The structural, biochemical, and computational data illuminate the principal functional components of the translation mechanism in mitochondria and provide the most complete description so far of the structure and function of the human mitoribosome. |
External links | bioRxiv / PubMed:37503168 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.21 - 2.98 Å |
Structure data | EMDB-13980: Human mitochondrial ribosome at 2.2 A resolution EMDB-13981, PDB-7qi5: EMDB-13982, PDB-7qi6: |
Chemicals | ChemComp-NAD: ChemComp-SPM: ChemComp-SPD: ChemComp-MG: ChemComp-K: ChemComp-ZN: ChemComp-FES: ChemComp-ATP: ChemComp-GDP: ChemComp-PUT: ChemComp-VAL: ChemComp-HOH: |
Source |
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Keywords | RIBOSOME / mitochondrial translation / tRNA / mRNA / 2Fe-2S clusters / polyamines / rRNA modifications / post-translation modifications / cryo EM |