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TitleHistone divergence in trypanosomes results in unique alterations to nucleosome structure.
Journal, issue, pagesNucleic Acids Res, Vol. 51, Issue 15, Page 7882-7899, Year 2023
Publish dateAug 25, 2023
AuthorsGauri Deák / Hannah Wapenaar / Gorka Sandoval / Ruofan Chen / Mark R D Taylor / Hayden Burdett / James A Watson / Maarten W Tuijtel / Shaun Webb / Marcus D Wilson /
PubMed AbstractEukaryotes have a multitude of diverse mechanisms for organising and using their genomes, but the histones that make up chromatin are highly conserved. Unusually, histones from kinetoplastids are ...Eukaryotes have a multitude of diverse mechanisms for organising and using their genomes, but the histones that make up chromatin are highly conserved. Unusually, histones from kinetoplastids are highly divergent. The structural and functional consequences of this variation are unknown. Here, we have biochemically and structurally characterised nucleosome core particles (NCPs) from the kinetoplastid parasite Trypanosoma brucei. A structure of the T. brucei NCP reveals that global histone architecture is conserved, but specific sequence alterations lead to distinct DNA and protein interaction interfaces. The T. brucei NCP is unstable and has weakened overall DNA binding. However, dramatic changes at the H2A-H2B interface introduce local reinforcement of DNA contacts. The T. brucei acidic patch has altered topology and is refractory to known binders, indicating that the nature of chromatin interactions in T. brucei may be unique. Overall, our results provide a detailed molecular basis for understanding evolutionary divergence in chromatin structure.
External linksNucleic Acids Res / PubMed:37427792 / PubMed Central
MethodsEM (single particle)
Resolution3.3 Å
Structure data

16777

8com

EMDB-16777, PDB-8com:
Structure of the Nucleosome Core Particle from Trypanosoma brucei
Method: EM (single particle) / Resolution: 3.3 Å

Source
  • trypanosoma brucei brucei treu927 (eukaryote)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN / Nucleosome Chromatin Parasite Trypanosome Kinetoplast

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