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-Structure paper
タイトル | Nuclear mRNPs are compact particles packaged with a network of proteins promoting RNA-RNA interactions. |
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ジャーナル・号・ページ | Genes Dev, Vol. 37, Issue 11-12, Page 505-517, Year 2023 |
掲載日 | 2023年6月1日 |
著者 | Fabien Bonneau / Jérôme Basquin / Barbara Steigenberger / Tillman Schäfer / Ingmar B Schäfer / Elena Conti / |
PubMed 要旨 | Messenger RNAs (mRNAs) are at the center of the central dogma of molecular biology. In eukaryotic cells, these long ribonucleic acid polymers do not exist as naked transcripts; rather, they associate ...Messenger RNAs (mRNAs) are at the center of the central dogma of molecular biology. In eukaryotic cells, these long ribonucleic acid polymers do not exist as naked transcripts; rather, they associate with mRNA-binding proteins to form messenger ribonucleoprotein (mRNP) complexes. Recently, global proteomic and transcriptomic studies have provided comprehensive inventories of mRNP components. However, knowledge of the molecular features of distinct mRNP populations has remained elusive. We purified endogenous nuclear mRNPs from by harnessing the mRNP biogenesis factors THO and Sub2 in biochemical procedures optimized to preserve the integrity of these transient ribonucleoprotein assemblies. We found that these mRNPs are compact particles that contain multiple copies of Yra1, an essential protein with RNA-annealing properties. To investigate their molecular and architectural organization, we used a combination of proteomics, RNA sequencing, cryo-electron microscopy, cross-linking mass spectrometry, structural models, and biochemical assays. Our findings indicate that yeast nuclear mRNPs are packaged around an intricate network of interconnected proteins capable of promoting RNA-RNA interactions via their positively charged intrinsically disordered regions. The evolutionary conservation of the major mRNA-packaging factor (yeast Yra1 and Aly/REF in metazoans) points toward a general paradigm governing nuclear mRNP packaging. |
リンク | Genes Dev / PubMed:37399331 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 11.0 Å |
構造データ | EMDB-16841: S.cerevisiae THO complex from endogenous nuclear mRNPs |
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